Bone Fracture Pre-Ischemic Stroke Exacerbates Ischemic Cerebral Injury in Mice
| Autor: | Lei Zhan, Shuai Kang, Zhengxi Li, Dingquan Zou, Liang Wang, Wan Zhu, Hua Su |
|---|---|
| Přispěvatelé: | Ahmad, Muzamil |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Male Pathology Middle Cerebral Artery Critical Care and Emergency Medicine lcsh:Medicine Tibia Fracture Pathology and Laboratory Medicine Inbred C57BL Vascular Medicine Transgenic Bone remodeling Brain Ischemia Brain ischemia Immunoenzyme Techniques White Blood Cells Mice 0302 clinical medicine Animal Cells Receptors Medicine and Health Sciences 2.1 Biological and endogenous factors Brain Damage Aetiology lcsh:Science Musculoskeletal System Immune Response Stroke Trauma Medicine Mammals Multidisciplinary Microglia medicine.anatomical_structure Neurology Bone Fracture Infarction Chemokine Vertebrates Cytokines Anatomy Cellular Types medicine.symptom Traumatic Injury Research Article medicine.medical_specialty General Science & Technology Cerebrovascular Diseases Immune Cells Knockout Immunology CX3C Chemokine Receptor 1 Enzyme-Linked Immunosorbent Assay Brain damage Rodents 03 medical and health sciences Signs and Symptoms Diagnostic Medicine medicine Animals cardiovascular diseases Bone Skeleton Neuroinflammation Ischemic Stroke Inflammation Behavior Blood Cells Tibia business.industry Animal Macrophages lcsh:R Organisms Neurosciences Biology and Life Sciences Cell Biology Bone fracture medicine.disease Brain Disorders 030104 developmental biology Brain Injuries Amniotes Disease Models CCR2 lcsh:Q business Fractures 030217 neurology & neurosurgery |
| Zdroj: | PloS one, vol 11, iss 4 Wang, L; Kang, S; Zou, D; Zhan, L; Li, Z; Zhu, W; et al.(2016). Bone fracture pre-ischemic stroke exacerbates ischemic cerebral injury in mice. PLoS ONE, 11(4), e0153835. doi: 10.1371/journal.pone.0153835. UCSF: Retrieved from: http://www.escholarship.org/uc/item/5r1877qs PLoS ONE PLoS ONE, Vol 11, Iss 4, p e0153835 (2016) |
| Popis: | © 2016 Wang et al.This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Ischemic stroke is a devastating complication of bone fracture. Bone fracture shortly after stroke enhances stroke injury by augmenting inflammation. We hypothesize that bone fracture shortly before ischemic stroke also exacerbates ischemic cerebral injury. Tibia fracture was performed 6 or 24 hours before permanent middle cerebral artery occlusion (pMCAO) on C57BL/6J mice or Ccr2RFP/+Cx3cr1GFP/+mice that have the RFP gene knocked into one allele of Ccr2 gene and GFP gene knocked into one allele of Cx3cr1 gene. Behavior was tested 3 days after pMCAO. Infarct volume, the number of CD68+cells, apoptotic neurons, bone marrow-derived macrophages (RFP+), and microgila (GFP+) in the peri-infarct region were quantified. Compared to mice subjected to pMCAO only, bone fracture 6 or 24 hours before pMCAO increased behavioral deficits, the infarct volume, and the number of CD68+cells and apoptotic neurons in the peri-infarct area. Both bone marrow-derived macrophages (CCR2+) and microglia (CX3CR1+) increased in the peri-infarct regions of mice subjected to bone fracture before pMCAO compared to stroke-only mice. The mice subjected to bone fracture 6 hours before pMCAO had more severe injury than mice that had bone fracture 24 hours before pMCAO. Our data showed that bone fracture shortly before stroke also increases neuroinflammation and exacerbates ischemic cerebral injury. Our findings suggest that inhibition of neuroinflammation or management of stroke risk factors before major bone surgery would be beneficial for patients who are likely to suffer from stroke. |
| Databáze: | OpenAIRE |
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