Immobilization of dextransucrase on functionalized TiO2 supports
| Autor: | Dušan N. Sredojević, Dejan Bezbradica, Jovan M. Nedeljković, Aleksandar D. Marinković, Slađana Davidović, Vesna Lazić, Suzana I. Dimitrijević Branković, Katarina Banjanac, Miona G. Miljković |
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| Rok vydání: | 2018 |
| Předmět: |
Immobilized enzyme
02 engineering and technology 01 natural sciences Biochemistry Dextransucrase chemistry.chemical_compound Structural Biology surface functionalization of TiO2 Thermal stability Molecular Biology enzyme immobilization 010405 organic chemistry Chemistry Ligand dextransucrase General Medicine Epoxy 021001 nanoscience & nanotechnology Charge-transfer complex 0104 chemical sciences visual_art visual_art.visual_art_medium Surface modification Glutaraldehyde 0210 nano-technology Nuclear chemistry |
| Zdroj: | International Journal of Biological Macromolecules |
| Popis: | The TiO2 based hybrid supports with different functional groups (amino, glutaraldehyde or epoxy) were prepared and their influence on immobilization of dextransucrase (DS) was studied. Novel synthetic route for surface modification of TiO2 with amino and glutaraldehyde groups was developed taking advantage of charge transfer complex (CTC) formation between surface Ti atoms and salicylate-type of ligand (5 aminosalicylic acid (5-ASA)). The proposed coordination of 5-ASA to the surface of TiO2 powder and optical properties of CTC was presented. The pristine TiO2 and amino functionalized TiO2 have higher sorption capacity for DS (12.6 and 12.0 mg g(-1), respectively) compared to glutaraldehyde and epoxy activated supports (9.6 and 9.8 mg g(-1) respectively). However, immobilized enzyme to either glutaraldehyde or epoxy functionalized TiO2 have almost two times higher expressed activities compared to pristine TiO2 support (258, 235 and 142 IU g(-1), respectively). Thermal stability of enzyme immobilized on glutaraldehyde and epoxy functionalized supports was studied at 40 degrees C, as well as operational stability under long-run working conditions in repeated cycles. After five cycles, DS imobilized on glutaraldehyde activated support retained almost 70% of its initial expressed activity, while, after five cycles, performance of DS immobilized on epoxy activated support was significantly lower (15%). |
| Databáze: | OpenAIRE |
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