Improved effect of pumpkin seed oil against the bisphenol-A adverse effects in male mice
| Autor: | M. Mahmoud El-Bamby, Eissa I. Fawzy, Aida I. El Makawy, H. Osama Elhamalawy |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
endocrine system medicine.medical_specialty Pumpkin seed oil DNA damage Bisphenol-A Health Toxicology and Mutagenesis Histopathology 010501 environmental sciences Toxicology medicine.disease_cause 01 natural sciences Article Andrology 03 medical and health sciences lcsh:RA1190-1270 Micronucleus test medicine Adverse effect Comet assay Pumpkin Seed Oil lcsh:Toxicology. Poisons 0105 earth and related environmental sciences urogenital system business.industry 030104 developmental biology medicine.anatomical_structure Bone marrow Genotoxicity business hormones hormone substitutes and hormone antagonists |
| Zdroj: | Toxicology Reports Toxicology Reports, Vol 5, Iss, Pp 857-863 (2018) |
| ISSN: | 2214-7500 |
| DOI: | 10.1016/j.toxrep.2018.08.014 |
| Popis: | The present study was conducted to evaluate the ameliorative role of pumpkin seed oil (PSO) against potential adverse effects of bisphenol-A (BPA) in male mice. BPA was administered to the mice orally at a dose of 50 mg/kg body weight once a day for 28 successive days. While, PSO was administered to the mice orally at 1 mL/kg b w either before, with or after treatment of BPA, once a day for 28 successive days. The studied parameters were DNA damage evaluation using comet assay in liver and testes cells and micronucleus test in bone marrow; and histopathological examination of liver and testes tissues. Results revealed that BPA induced DNA damage in tested cells and marked histopathological alterations in liver and testes. In contrast, PSO treatments alleviated DNA damage and improved the histopathological alterations in liver and testes tissues. Furthermore, administration of mice with the PSO before BPA treatment was the best regimen in the alleviation of the adverse effects of BPA, followed by administration of PSO after then with treatment of BPA. It can be concluded that PSO may has a protective role against BPA genotoxicity and histopathological alterations in male mice. Keywords: Pumpkin seed oil, Bisphenol-A, Genotoxicity, Micronucleus test, Comet assay, Histopathology |
| Databáze: | OpenAIRE |
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