Temporal differences in the activation of three classes of non-transmembrane protein tyrosine kinases following B-cell antigen receptor surface engagement
| Autor: | Joseph Fargnoli, Owen N. Witte, Sandra J. Saouaf, Joseph B. Bolen, R. B. Rowley, S. A. Kut, Anne L. Burkhardt, Satoshi Tsukada, Sandeep Mahajan |
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| Rok vydání: | 1994 |
| Předmět: |
Time Factors
Molecular Sequence Data B-cell receptor Receptors Antigen B-Cell Syk Protein tyrosine phosphatase Receptor tyrosine kinase Cell Line Mice chemistry.chemical_compound LYN hemic and lymphatic diseases Animals Amino Acid Sequence Phosphotyrosine Conserved Sequence B-Lymphocytes Multidisciplinary biology Tyrosine phosphorylation Protein-Tyrosine Kinases Phosphoproteins Molecular biology Enzyme Activation Kinetics chemistry Immunoglobulin G ROR1 biology.protein Tyrosine Tyrosine kinase Research Article |
| Zdroj: | Proceedings of the National Academy of Sciences. 91:9524-9528 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.91.20.9524 |
| Popis: | We evaluated in WEHI 231 B cells the time-dependent responses of Lyn, Blk, Btk, Syk, and three members of the Jak family of protein tyrosine kinases following antibody-mediated surface engagement of the B-cell antigen receptor. Our results show that the enzyme activities of Lyn and Blk were stimulated within seconds of antigen receptor engagement and correlated with the initial tyrosine phosphorylation of the Ig alpha and Ig beta subunits of the B-cell antigen receptor. Btk enzyme activity was also transiently stimulated and was maximal at approximately 5 min after B-cell receptor surface binding. Syk activity gradually increased to a maximum at 10-30 min following receptor ligation and was found to parallel the association of Syk with the tyrosine phosphorylated Ig alpha and Ig beta subunits of the receptor. While the specific activities of the Jak1, Jak2, and Tyk2 protein tyrosine kinases were unaltered following B-cell receptor ligation, the abundance of Jak1 and Jak2 were increased 3- to 4-fold within 10 min of receptor engagement. These results demonstrate that multiple families of non-transmembrane protein tyrosine kinases are temporally regulated during the process of B-cell antigen receptor-initiated intracellular signal transduction. |
| Databáze: | OpenAIRE |
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