| Autor: |
Hyeong-Min Lee, M. Bram Kuijer, Blanes, Nerea Ruiz, Clark, Ellen P., Aita, Megumi, Arjona, Lorena Galiano, Kokot, Agnieszka, Sciaky, Noah, Simon, Jeremy M., Sanchita Bhatnagar, Philpot, Benjamin D., Cerase, Andrea |
| Rok vydání: |
2020 |
| DOI: |
10.6084/m9.figshare.13219490.v1 |
| Popis: |
Additional file 1: Supplementary figure 1. Compound validation under different treatment regimens. A) AG490 validation in 6-well plate format. Relative Luminescence Units (RLU) is shown in function of the drug treatment for the clone Xi8. B) qRT-PCR of MeCP2, luciferase, Xist and Rnf12. Left: single treatment for the 72 hours regimen for clone Xi8. Right: double treatment regimen for 72 hr each for clone Xi8. Bars indicate standard error of the mean (SEM). * indicates statistical significance, p ≤ 0.05, n = 3 per genes, average of two independent assays is shown) (C) Top: Representative fluorescent immunostaining images of cortical neurons in presence or absence of the compounds with double treatment (i.e. twice for 72 hr). Bottom: Quantitative analysis of number of GFP positive cells. The % of GFP positive cells increased in the presence of AG490 and Jaki with a double treatment regimen. Bars indicate standard error of the mean (SEM), samples are color-coded, concentrations are shown. * indicates statistical significance, p ≤ 0.05, n = 4 per compound and dose, average of two independent assays is shown). |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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