Applicability of a Voltammetric Assay Based on the Electroreduction of Oxygen to Evaluate the Antioxidant Capacity of Pequi (Caryocar brasiliense Camb.) Pulp
| Autor: | Letícia Rocha Ferreira, Edilton de Souza Barcellos, Flávia de Abreu Pinheiro, Edimar Aparecida Filomeno Fontes, Astréa Filomena de Souza Silva, Júnio G. Silva, Leonardo Luiz Okumura |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Antioxidant
DPPH medicine.medical_treatment Trolox equivalent antioxidant capacity antioxidant capacity engineering.material 01 natural sciences pequi chemistry.chemical_compound Caryocar brasiliense 0404 agricultural biotechnology medicine Gallic acid Food science ABTS bioactive compounds biology food analysis Pulp (paper) 010401 analytical chemistry 04 agricultural and veterinary sciences General Chemistry biology.organism_classification 040401 food science 0104 chemical sciences chemistry electrochemical techniques engineering Trolox |
| Zdroj: | Journal of the Brazilian Chemical Society, Volume: 29, Issue: 8, Pages: 1653-1662, Published: AUG 2018 Journal of the Brazilian Chemical Society v.29 n.8 2018 Journal of the Brazilian Chemical Society Sociedade Brasileira de Química (SBQ) instacron:SBQ LOCUS Repositório Institucional da UFV Universidade Federal de Viçosa (UFV) instacron:UFV |
| Popis: | This study aimed to use a differential pulse voltammetric (DPV) assay, based on the electroreduction of oxygen, to evaluate the antioxidant capacity of pequi pulp. The results were expressed in coefficient of antioxidant capacity (K), at different pH values and in trolox equivalent antioxidant capacity (TEAC), and were compared to the 2,2’-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) spectrophotometric assays. The principal component analysis showed higher K for pequi pulp extract and pH tending to 7.15. The antioxidant capacity was related to the presence of gallic acid in its composition. Regarding the TEAC value, the best result was obtained by the DPV test (53.43 ± 5.14 µmol trolox g-1 pulp). The pequi pulp showed antioxidant capacity, compared to the standard antioxidant compounds evaluated. It was concluded that the voltammetric protocol was efficient to assess the total antioxidant capacity of pequi pulp and can be an alternative to conventional spectrophotometric assays, for the analysis of food matrices. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|