The role of CTHRC1 in hair follicle regenerative capacity restored by plantar dermis homogenate
| Autor: | Xiaobing Fu, Yuzhen Wang, Xingyu Yuan, Enhejirigala, Yijie Zhang, Sha Huang, Jianjun Li, Xianlan Duan, Bin Yao, Wei Song, Zhao Li |
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| Rok vydání: | 2021 |
| Předmět: |
Mice
Knockout Extracellular Matrix Proteins Chemistry Regeneration (biology) Biophysics Spheroid Dermis Cell Biology Matrix (biology) Hair follicle Biochemistry Cell biology Mice Inbred C57BL Mice medicine.anatomical_structure Tissue engineering medicine Animals Hair Follicle Molecular Biology Organ regeneration |
| Zdroj: | Biochemical and Biophysical Research Communications. 571:14-19 |
| ISSN: | 0006-291X |
| Popis: | Restoration of hair follicle (HF) regenerative capacity is the cornerstone in tissue engineering for the loss of regenerative capacity during in vitro expansion of skin-derived precursors (SKPs). Microenvironmental cues facilitated tissue or organ regeneration offers a potential strategy to overcome this difficulty. In our previous work, plantar dermis matrix homogenate (PD) has been proved to induce sweat glands regeneration both in vivo and in vitro. Here, we found PD also restore regenerative capacity of culture impaired HF spheroids (IHFS). Further, followed by our previous iTRAQ results, the CTHRC1 was identified as a potential regulator in PD facilitated restorative effects in HF regeneration. Knockout of Cthrc1 impaired HF regenerative capacity in spheroids, decreased the diameter of HF in 28 postnatal days mice and shortened invagination of HF bud in 18 days of gestation mice. In IHFS and Cthrc1−/− spheroids, PD partially restored HF regenerative capacity while Cthrc1−/− PD (PDKO) has less or no effect. Taken together, PD is an effective microenvironmental cues for HF regenerative capacity restoration and CTHRC1 played an important role in HF regeneration. |
| Databáze: | OpenAIRE |
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