Electricity-Producing Staphylococcus Epidermidis Counteracts Cutibacterium Acnes
| Autor: | Sunita Keshari, Ming-Fa Hsieh, Arun Balasubramaniam, Supitchaya Traisaeng, Deron R. Herr, Shinta Marito, Prakoso Adi, Chun Ming Huang, Do Thi Tra My |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Lysis Science 030106 microbiology Gene Expression Pathogenesis Microbiology Article Polyethylene Glycols Mice Surface-Active Agents 03 medical and health sciences Cyclophilin A Bacterial Proteins Electricity In vivo Staphylococcus epidermidis Acne Vulgaris Antibiosis PEG ratio Animals Intradermal injection Electrodes Skin Mice Inbred ICR Multidisciplinary Innate immune system biology Chemistry Ear Propionibacteriaceae biology.organism_classification Coculture Techniques In vitro Culture Media Disease Models Animal 030104 developmental biology Medicine Female Laurates |
| Zdroj: | Scientific Reports, Vol 11, Iss 1, Pp 1-11 (2021) Scientific Reports |
| Popis: | Staphylococcus epidermidis (S. epidermidis) ATCC 12228 was incubated with 2% polyethylene glycol (PEG)-8 Laurate to yield electricity which was measured by a voltage difference between electrodes. Production of electron was validated by a Ferrozine assay. The anti-Cutibacterium acnes (C. acnes) activity of electrogenic S. epidermidis was assessed in vitro and in vivo. The voltage change (~ 4.4 mV) reached a peak 60 minutes after pipetting S. epidermidis plus 2% PEG-8 Laurate onto anodes. The electricity produced by S. epidermidis caused significant growth attenuation and cell lysis of C. acnes. Intradermal injection of C. acnes and S. epidermidis plus PEG-8 Laurate into the mouse ear considerably suppressed the growth of C. acnes. This suppressive effect was noticeably reversed when cyclophilin A of S. epidermidis was inhibited, indicating the essential role of cyclophilin A in electricity production of S. epidermidis against C. acnes. In summary, we demonstrate for the first time that skin S. epidermidis, in the presence of PEG-8 Laurate, can mediate cyclophilin A to elicit an electrical current that has anti-C. acnes effects. Electricity generated by S. epidermidis may confer immediate innate immunity in acne lesions to rein in the overgrowth of C. acnes at the onset of acne vulgaris. |
| Databáze: | OpenAIRE |
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