Eye-specific gene expression following embryonic ethanol exposure in zebrafish: Roles for heat shock factor 1
| Autor: | Deborah L. Stenkamp, Bhavani Kashyap, Laurel Pegorsch, Eric A. Shelden, Ruth A. Frey, Chi Sun |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Embryo
Nonmammalian Morpholino Eye Toxicology Microphthalmia Article Heat Shock Transcription Factors Heat shock protein Cellular stress response Gene expression medicine Animals Microphthalmos HSF1 Zebrafish Oligonucleotide Array Sequence Analysis Ethanol biology Gene Expression Profiling Gene Expression Regulation Developmental Zebrafish Proteins biology.organism_classification medicine.disease Molecular biology eye diseases Heat shock factor Transcription Factors |
| Zdroj: | Reproductive Toxicology. 43:111-124 |
| ISSN: | 0890-6238 |
| DOI: | 10.1016/j.reprotox.2013.12.002 |
| Popis: | The mechanisms through which ethanol exposure results in developmental defects remain unclear. We used the zebrafish model to elucidate eye-specific mechanisms that underlie ethanol-mediated microphthalmia (reduced eye size), through time-series microarray analysis of gene expression within eyes of embryos exposed to 1.5% ethanol. 62 genes were differentially expressed (DE) in ethanol-treated as compared to control eyes sampled during retinal neurogenesis (24-48 hours post-fertilization). The EDGE (extraction of differential gene expression) algorithm identified >3000 genes DE over developmental time in ethanol-exposed eyes as compared to controls. The DE lists included several genes indicating a mis-regulated cellular stress response due to ethanol exposure. Combined treatment with sub-threshold levels of ethanol and a morpholino targeting heat shock factor 1 mRNA resulted in microphthalmia, suggesting convergent molecular pathways. Thermal preconditioning partially prevented ethanol-mediated microphthalmia while maintaining Hsf-1 expression. These data suggest roles for reduced Hsf-1 in mediating microphthalmic effects of embryonic ethanol exposure. |
| Databáze: | OpenAIRE |
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