Antitumor effect of TW-37, a BH3 mimetic in human oral cancer
| Autor: | Ji Hoon Kim, Minjung Jung, Sung-Dae Cho, Ji-Ae Shin, Min-Hye Ahn, Chi-Hyun Ahn, Su-Jung Choi, Kyoung-Ok Hong, Won Woo Lee, Bohwan Jin, Yun Chan Jung, Neeti Swarup |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
education.field_of_study
lcsh:R5-920 Cell growth Chemistry Research Oral cancer Population Cell Apoptosis chemistry.chemical_compound medicine.anatomical_structure TW-37 lcsh:Biology (General) Cell culture In vivo Annexin medicine Cancer research Bcl-2 Propidium iodide education lcsh:Medicine (General) lcsh:QH301-705.5 |
| Zdroj: | Laboratory Animal Research, Vol 35, Iss 1, Pp 1-8 (2019) Laboratory Animal Research |
| ISSN: | 2233-7660 |
| Popis: | TW-37 is a small molecule B cell lymphoma-2 (Bcl-2) homology 3 mimetic with potential anticancer activities. However, the in vivo anti-cancer effect of TW-37 in human oral cancer has not been properly studied yet. Here, we attempted to confirm antitumor activity of TW37 in human oral cancer. TW-37 significantly inhibited cell proliferation and increased the number of dead cells in MC-3 and HSC-3 human oral cancer cell lines. TW-37 enhanced apoptosis of both cell lines evidenced by annexin V/propidium iodide double staining, sub-G1population analysis and the detection of cleaved poly (ADP-ribose) polymerase and caspase-3. In addition, TW-37 markedly downregulated the expression of Bcl-2 protein, while not affecting Bcl-xL or myeloid cell leukemia-1. In vivo, TW-37 inhibited tumor growth in a nude mice xenograft model without any significant liver and kidney toxicities. Collectively, these data reveal that TW-37 may be a promising small molecule to inhibit human oral cancer. |
| Databáze: | OpenAIRE |
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