Temporal Role of Sertoli Cell Androgen Receptor Expression in Spermatogenic Development
| Autor: | Charles M. Allan, Rasmani Hazra, David J. Handelsman, Lisa Corcoran, Mat Robson, Dannielle H. Upton, Kirsten J. McTavish |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Male
endocrine system medicine.medical_specialty Transgene Gene Expression Lumen (anatomy) Mice Transgenic Biology Mice Endocrinology Seminal vesicle Spermatocytes Internal medicine Testis medicine Animals Humans Testosterone Spermatogenesis Molecular Biology Original Research Serum testosterone Messenger RNA Sertoli Cells Gene Expression Regulation Developmental Organ Size General Medicine Seminiferous Tubules Sertoli cell Spermatogonia Rats Androgen receptor medicine.anatomical_structure Receptors Androgen Female Follicle Stimulating Hormone |
| Zdroj: | Molecular Endocrinology. 27:12-24 |
| ISSN: | 1944-9917 0888-8809 |
| DOI: | 10.1210/me.2012-1219 |
| Popis: | Sertoli cell (SC) androgen receptor (AR) activity is vital for spermatogenesis. We created a unique gain-of-function transgenic (Tg) mouse model to determine the temporal role of SCAR expression in testicular development. The SC-specific rat Abpa promoter directed human Tg AR [Tg SC-specific AR (TgSCAR)] expression, providing strong premature postnatal AR immunolocalized to SC nuclei. Independent Tg lines revealed that TgSCAR dose dependently reduced postnatal and mature testis size (to 60% normal), whereas androgen-dependent mature seminal vesicle weights and serum testosterone levels remained normal. Total SC numbers were reduced in developing and mature TgSCAR testes, despite normal or higher Fshr mRNA and circulating FSH levels. Postnatal TgSCAR testes exhibited elevated levels of AR-regulated Rhox5 and Spinlw1 transcripts, and precocious SC function was demonstrated by early seminiferous tubular lumen formation and up-regulated expression of crucial SC tight-junction (Cldn11 and Tjp1) and phagocytic (Elmo1) transcripts. Early postnatal Amh expression was elevated but declined to normal levels in peripubertal-pubertal TgSCAR vs. control testes, indicating differential age-related regulation featuring AR-independent Amh down-regulation. TgSCAR induced premature postnatal spermatogenic development, shown by increased levels of meiotic (Dmc1 and Spo11) and postmeiotic (Capza3 and Prm1) germ cell transcripts, elevated meiotic-postmeiotic germ:Sertoli cell ratios, and accelerated spermatid development. Meiotic germ:Sertoli cell ratios were further increased in adult TgSCAR mice, indicating predominant SCAR-mediated control of meiotic development. However, postmeiotic germ:Sertoli cell ratios declined below normal. Our unique TgSCAR paradigm reveals that atypical SC-specific temporal AR expression provides a direct molecular mechanism for induction of precocious testicular development, leading to reduced adult testis size and decreased postmeiotic development. |
| Databáze: | OpenAIRE |
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