Enhancement of Lindane-induced Liver Oxidative Stress and Hepatotoxicity by Thyroid Hormone is Reduced by Gadolinium Chloride
| Autor: | Leandro Giavarotti, Eduardo A. Garcia, Virginia Fernández, Alessandra F. Lima, Karin A. Simon-Giavarotti, Virginia Junqueira, Adriano M. Veridiano, Lígia Ferreira Gomes, Luis A. Videla, Oswaldo Alves Mora |
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| Rok vydání: | 2002 |
| Předmět: |
Male
medicine.medical_specialty Kupffer Cells Gadolinium medicine.disease_cause Hyperthyroidism Biochemistry Rats Sprague-Dawley chemistry.chemical_compound Cytochrome P-450 Enzyme System Superoxides Internal medicine medicine Animals NADPH-Ferrihemoprotein Reductase Liver injury Chemistry Thyroid General Medicine Catalase medicine.disease Rats Gadolinium chloride Oxidative Stress Endocrinology medicine.anatomical_structure Liver Microsomes Liver Triiodothyronine Hepatocellular injury Chemical and Drug Induced Liver Injury Energy Metabolism Lindane Hexachlorocyclohexane Oxidative stress Hormone |
| Zdroj: | FREE RADICAL RESEARCH Artículos CONICYT CONICYT Chile instacron:CONICYT |
| ISSN: | 1029-2470 1071-5762 |
| DOI: | 10.1080/1071576021000028280 |
| Popis: | The role of Kupffer cells in the hepatocellular injury and oxidative stress induced by lindane (20 mg/kg; 24h) in hyperthyroid rats (daily doses of 0.1 mg L-3,3',5-triiodothyronine (T3)/kg for three consecutive days) was assessed by the simultaneous administration of gadolinium chloride (GdCl3; 2 doses of 10mg/kg on alternate days). Hyperthyroid animals treated with lindane exhibit enhanced liver microsomal superoxide radical (O2.-) production and NADPH cytochrome c reductase activity, with lower levels of cytochrome P450, superoxide dismutase (SOD) and catalase activity, and glutathione (GSH) content over control values. These changes are paralleled by a substantial increase in the lipid peroxidation potential of the liver and in the O2.- generation/ SOD activity ratio, thus evidencing a higher oxidative stress status that correlates with the development of liver injury characterized by neutrophil infiltration and necrosis. Kupffer cell inactivation by GdCl3 suppresses liver injury in lindane/T3-treated rats with normalization of altered oxidative stress-related parameters, excepting the reduction in the content of GSH and in catalase activity. It is concluded that lindane hepatotoxicity in hyperthyroid state, that comprises an enhancement in the oxidative stress status of the liver, is largely dependent on Kupffer cell function, which may involve generation of mediators leading to pro-oxidant and inflammatory processes. |
| Databáze: | OpenAIRE |
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