Imprinted Expression of Neuronatin from Modified BAC Transgenes Reveals Regulation by Distinct and Distant Enhancers
| Autor: | Samuel Aparicio, Katharine L. Arney, Kathy Hilton, Sheila C. Barton, M. Azim Surani, Sanjeev Khosla, Rosalind M. John, Kelvin Hawker, Justin F. X. Ainscough |
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| Rok vydání: | 2001 |
| Předmět: |
Male
Chromosomes Artificial Bacterial BAC transgenesis Neuronatin Mice Transgenic Nerve Tissue Proteins Locus (genetics) Choristoma Biology Genomic Imprinting Mice 03 medical and health sciences 0302 clinical medicine Animals RNA Messenger Transgenes Cloning Molecular Allele Promoter Regions Genetic Enhancer Molecular Biology Gene Alleles In Situ Hybridization 030304 developmental biology Genetics 0303 health sciences Intron Membrane Proteins Cell Biology Introns Antisense RNA Enhancer Elements Genetic Gene Expression Regulation enhancers Female regulatory elements Genomic imprinting 030217 neurology & neurosurgery Plasmids Developmental Biology |
| Zdroj: | ResearcherID |
| ISSN: | 0012-1606 |
| Popis: | Neuronatin (Nnat) is an imprinted gene that is expressed exclusively from the paternal allele while the maternal allele is silent and methylated. The Nnat locus exhibits some unique features compared with other imprinted domains. Unlike the majority of imprinted genes, which are organised in clusters and coordinately regulated, Nnat does not appear to be closely linked to other imprinted genes. Also unusually, Nnat is located within an 8-kb intron of the Bc10 gene, which generates a biallelically expressed, antisense transcript. A similar organisation is conserved at the human NNAT locus on chromosome 20. Nnat expression is first detected at E8.5 in rhombomeres 3 and 5, and subsequently, expression is widespread within postmitotic neuronal tissues. Using modified BAC transgenes, we show that imprinted expression of Nnat at ectopic sites requires, at most, an 80-kb region around the gene. Furthermore, reporter transgenes reveal distinct and dispersed cis-regulatory elements that direct tissue-specific expression and these are predominantly upstream of the region that confers allele-specific expression. |
| Databáze: | OpenAIRE |
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