Giemsa staining of the sites replicating DNA early in human lymphocyte chromosomes

Autor:	My.A. Kim, R. Johannsmann, Karl-Heinz Grzeschik
Rok vydání:	1975
Předmět:	DNA Replication Autosome DNA replication Mitosis Biology Azure Stains Molecular biology Chromosomes Giemsa stain Staining chemistry.chemical_compound chemistry Phenothiazines Genetics Homologous chromosome Humans Female Lymphocytes Molecular Biology Metaphase Genetics (clinical) DNA
Zdroj:	Cytogenetic and Genome Research. 15:363-371
ISSN:	1424-859X 1424-8581
DOI:	10.1159/000130535
Popis:	A timetable for the initiation of DNA replication in human lymphocyte chromosomes has been established by a technique which allows detection of areas of chromosomes replicating at a given interval of the S-phase. The resolution of the method, using 33258 Hoechst-Giemsa staining, is more refined than that obtained with 3H-thymidine autoradiography. Early replicating regions coincide with R-bands. The timetable is rather coarse since replication may start asynchronously in the same region of homologous autosomes of the same metaphase and since even the sequence of bands appearing on individual chromosomes sometimes deviates from the rule.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::159b20f12d2791a80e9329b06b1d42b3 https://doi.org/10.1159/000130535 Zobrazit plný text záznamu