Asporin stably expressed in the surface layer of mandibular condylar cartilage and augmented in the deeper layer with age
| Autor: | Satoshi Wada, Kanako Itohiya, Yutaka Miyamoto, Hiroyuki Kanzaki, Yoshiki Nakamura, Sari Tsuruoka, Yoshiki Hamada, Kenichi Kumagai |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
TGF-β Pathology medicine.medical_specialty lcsh:Diseases of the musculoskeletal system TP tibial proliferating layer Endocrinology Diabetes and Metabolism MCC mandibular condylar cartilage Article 03 medical and health sciences 0302 clinical medicine MF mandibular fibrous layer Gene expression medicine TH tibial hypertrophic layer Orthopedics and Sports Medicine Tibia MP mandibular proliferating layer Asporin MH mandibular hypertrophic layer Chemistry Microarray analysis techniques Mandibular condylar cartilage Cartilage TR tibial reserve layer Endochondral growth food and beverages Staining 030104 developmental biology medicine.anatomical_structure Immunohistochemistry Growth plate lcsh:RC925-935 030217 neurology & neurosurgery Transforming growth factor |
| Zdroj: | Bone Reports, Vol 7, Iss C, Pp 41-50 (2017) Bone Reports |
| ISSN: | 2352-1872 |
| Popis: | Mandibular condylar cartilage (MCC) exhibits dual roles both articular cartilage and growth center. Of many growth factors, TGF-β has been implicated in the growth of articular cartilage including MCC. Recently, Asporin, decoy to TGF-β, was discovered and it blocks TGF-β signaling. Asporin is expressed in a variety of tissues including osteoarthritic articular cartilage, though there was no report of Asporin expression in MCC. In the present study, we investigated the temporal and spatial expression of Asporin in MCC. Gene expression profile of MCC and epiphyseal cartilage in tibia of 5 weeks old ICR mice were firstly compared with microarray analysis using the laser capture microdissected samples. Variance of gene expression was further confirmed by real-time RT-PCR and immunohistochemical staining at 1,3,10, and 20 weeks old. TGF-β and its signaling molecule, phosphorylated Smad-2/3 (p-Smad2/3), were also examined by immunohistochemical staining. Microarray analysis revealed that Asporin was highly expressed in MCC. Real-time RT-PCR analysis confirmed that the fibrous layer of MCC exhibited stable higher Asporin expression at any time points as compared to epiphyseal cartilage. This was also observed in immunohistochemical staining. Deeper layer in MCC augmented Asporin expression with age. Whereas, TGF-β was stably highly observed in the layer. The fibrous layer of MCC exhibited weak staining of p-Smad2/3, though the proliferating layer of MCC was strongly stained as compared to epiphyseal cartilage of tibia at early time point. Consistent with the increase of Asporin expression in the deeper layer of MCC, the intensity of p-Smad-2/3 staining was decreased with age. In conclusion, we discovered that Asporin was stably expressed at the fibrous layer of MCC, which makes it possible to manage both articular cartilage and growth center at the same time. Highlights • Asporin gene and protein were highly expressed in mandibular condylar cartilage as compared to tibial epiphyseal cartilage. • Asporin in mandibular condylar cartilage was augmented with age. • TGF-β signaling is suppressed by augmented Asporin and decreased TGF-β production in mandibular condylar cartilage. |
| Databáze: | OpenAIRE |
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