Development of an optical fiber-based redox monitoring system for tissue metabolism
| Autor: | Wen Qi Zhang, Alexandra Sorvina, Janna L. Morrison, Jack R. T. Darby, Doug A. Brooks, Sally E. Plush, Shahraam Afshar Vahid |
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| Přispěvatelé: | Zhang, Wen Qi, Sorvina, Alexandra, Morrison, Janna L., Darby, Jack R.T., Brooks, Doug A., Plush, Sally E., Afshar Vahid, Shahraam |
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
optical fiber
General Engineering General Physics and Astronomy General Chemistry NAD General Biochemistry Genetics and Molecular Biology Microscopy Fluorescence Multiphoton redox Flavin-Adenine Dinucleotide General Materials Science fluorescence two-photon microscopy Oxidation-Reduction Optical Fibers |
| Popis: | An optical redox ratio can potentially be used to report on the dynamics of cell and tissue metabolism and define altered metabolic conditions for different pathologies. While there are methods to measure the optical redox ratio, they are not particularly suited to real-time in situ or in vivo analysis. Here, we have developed a fiber-optic system to measure redox ratios in cells and tissues and two mathematical models to enable real-time, in vivo redox measurements. The optical redox ratios in tissue explants are correlated directly with endogenous NADH/FAD fluorescence emissions. We apply the mathematical models to the two-photon microscopy data and show consistent results. We also used our fiber-optic system to measure redox in different tissues and show consistent results between the two models, hence demonstrating proof-of-principle. This innovative redox monitoring system will have practical applications for defining different metabolic disease states Refereed/Peer-reviewed |
| Databáze: | OpenAIRE |
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