Determination of steroid hormones in grey seal (Halichoerus grypus) blood plasma using convergence chromatography tandem mass spectrometry

Autor: Shannen Thora Lea Sait, Vaida Survilienė, Mart Jüssi, Susana V. Gonzalez, Tomasz Maciej Ciesielski, Bjørn Munro Jenssen, Alexandros G. Asimakopoulos
Rok vydání: 2023
Předmět:
Zdroj: Sait, S T L, Survilienė, V, Jüssi, M, Gonzalez, S V, Ciesielski, T M, Jenssen, B M & Asimakopoulos, A G 2023, ' Determination of steroid hormones in grey seal ( Halichoerus grypus ) blood plasma using convergence chromatography tandem mass spectrometry ', Talanta, vol. 254, 124109 . https://doi.org/10.1016/j.talanta.2022.124109
Talanta, Amsterdam : Elsevier B.V., 2023, vol. 254, art. no.124109, p. [1-13]
Talanta: The International Journal of Pure and Applied Analytical Chemistry
ISSN: 0039-9140
1873-3573
Popis: A hybrid solid phase extraction (HybridSPE) protocol tailored to ultra-performance convergence chromatography tandem mass spectrometry (UPC2-MS/MS) was developed for the determination of 19 steroid hormones in grey seal (Halichoerus grypus) blood plasma. In this study, the protocol demonstrated acceptable absolute recoveries ranging from 33 to 90%. The chromatographic separation was carried out using a gradient elution program with a total run time of 5 min. The method repeatability ranged from 1.9% to 24% for most target analytes and the method limits of quantification (mLOQs) ranged from 0.03 to 1.67 ng/mL. A total of 9 plasma samples were analysed to demonstrate the applicability of the developed method in blood plasma from a wildlife species. A total of 13 steroid hormones were quantified in grey seal pup plasma. The most prevalent steroids: cortisol, cortisone, corticosterone, 11-deoxycortisol, progesterone and 17α-hydroxyprogesterone were detected at concentrations in the range of 9.09–40.1, 7.10–24.2, 0.742–10.7, 1.06–5.72, 0.38–4.38 and
Databáze: OpenAIRE
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