Reply to Steinman and Wang: Involvement of the p150 isoform of the RNA editing enzyme ADAR1 in embryonic lethality

Autor:	Simone Visosky Ward, Cyril X. George, Michael B. A. Oldstone, Charles E. Samuel
Rok vydání:	2011
Předmět:	Gene isoform Genetics chemistry.chemical_classification animal structures Multidisciplinary Gene targeting Biology Embryonic stem cell Molecular biology Exon Enzyme Adenosine deaminase chemistry RNA editing Transcription (biology) embryonic structures biology.protein Letters
Zdroj:	Proceedings of the National Academy of Sciences. 108
ISSN:	1091-6490 0027-8424
Popis:	In their letter, Steinman and Wang (1) raise the possibility that p110 expression may be affected by specific gene targeting of the p150 isoform of adenosine deaminase 1 (ADAR1), similar to the concerns raised in the letter by Matthaei et al. (2). Although Steinman and Wang acknowledge that the levels of the constitutive exon 1B-containing transcript encoding the p110 isoform of ADAR1 were similar in both p150+/− and p150−/− MEF cells derived from gene-targeted embryos compared with MEF cells derived from WT embryos as shown in our article (3), they suggest that protein expression may have been affected as a result of antisense ADAR1 transcription from the PGK promoter. However, they correctly indicate that there is no evidence to support this possibility.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::158dd51f52061e155a793e792a4f2bbd https://doi.org/10.1073/pnas.1105352108 Zobrazit plný text záznamu