CD24, CD44 and EpCAM enrich for tumour-initiating cells in a newly established patient-derived xenograft of nasopharyngeal carcinoma
| Autor: | Norazlin Abdul Aziz, Alan Soo Beng Khoo, Suat-Cheng Peh, Kitson Liew, Lu Ping Tan, Susan Ling Ling Hoe, Yoon Ming Chin, Sin-Yeang Teow, Cheng Eng Koay, Tai Lin Chu, Ching Ching Ng, Fazlyn Reeny Abdul Razak, Nurul Ashikin Mohamed Shahrehan, Kwok Wai Lo, Noor Kaslina Mohd Kornain, Munirah Ahmad |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty Transplantation Heterologous Cell lcsh:Medicine Mice SCID Article Kruppel-Like Factor 4 03 medical and health sciences 0302 clinical medicine Mice Inbred NOD Cell Line Tumor Biomarkers Tumor otorhinolaryngologic diseases medicine Animals Humans lcsh:Science Mice Knockout Nasopharyngeal Carcinoma Multidisciplinary biology CD24 Cell growth lcsh:R CD44 CD24 Antigen Nasopharyngeal Neoplasms Epithelial Cell Adhesion Molecule medicine.disease Transplantation stomatognathic diseases Hyaluronan Receptors 030104 developmental biology medicine.anatomical_structure Nasopharyngeal carcinoma Cell culture KLF4 030220 oncology & carcinogenesis Neoplastic Stem Cells Cancer research biology.protein lcsh:Q Female |
| Zdroj: | Scientific Reports Scientific Reports, Vol 7, Iss 1, Pp 1-13 (2017) |
| ISSN: | 2045-2322 |
| Popis: | Subpopulations of nasopharyngeal carcinoma (NPC) contain cells with differential tumourigenic properties. Our study evaluates the tumourigenic potential of CD24, CD44, EpCAM and combination of EpCAM/CD44 cells in NPC. CD44br and EpCAMbr cells enriched for higher S-phase cell content, faster-growing tumourigenic cells leading to tumours with larger volume and higher mitotic figures. Although CD44br and EpCAMbr cells significantly enriched for tumour-initiating cells (TICs), all cells could retain self-renewal property for at least four generations. Compared to CD44 marker alone, EpCAM/CD44dbr marker did not enhance for cells with faster-growing ability or higher TIC frequency. Cells expressing high CD44 or EpCAM had lower KLF4 and p21 in NPC subpopulations. KLF4-overexpressed EpCAMbr cells had slower growth while Kenpaullone inhibition of KLF4 transcription increased in vitro cell proliferation. Compared to non-NPC, NPC specimens had increased expression of EPCAM, of which tumours from advanced stage of NPC had higher expression. Together, our study provides evidence that EpCAM is a potentially important marker in NPC. |
| Databáze: | OpenAIRE |
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