Gastroprotective and Antioxidative Effects of the Traditional Thai Polyherbal Formula Phy-Blica-D against Ethanol-Induced Gastric Ulcers in Rats
| Autor: | Sineenart Sanpinit, Piriya Chonsut, Chuchard Punsawad, Palika Wetchakul |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Male
antioxidant activity Administration Oral gastroprotective effect Article Antioxidants herbal formula oxidative stress ethanol-induced gastric ulcer Phy-Blica-D Animals TX341-641 Stomach Ulcer Rats Wistar Nutrition and Dietetics Plants Medicinal Ethanol Nutrition. Foods and food supply Plant Extracts Superoxide Dismutase Anti-Ulcer Agents Catalase Thailand Oxidative Stress Acute Disease Food Science Phytotherapy |
| Zdroj: | Nutrients Nutrients; Volume 14; Issue 1; Pages: 172 Nutrients, Vol 14, Iss 172, p 172 (2022) |
| ISSN: | 2072-6643 |
| Popis: | Phy-Blica-D is a traditional Thai polyherbal formula that has reduced oxidative stress in non-communicable diseases. However, evidence supporting the gastroprotective effects of Phy-Blica-D has not been previously reported. Therefore, this study aimed to evaluate the gastroprotective effects of Phy-Blica-D against gastric ulcers in rats and investigate the potential underlying mechanism. To estimate the possible mechanisms of action, we examined the levels of oxidative stress markers, such as reactive oxygen species (ROS) and malondialdehyde (MDA), as well as antioxidant enzymes, including catalase (CAT), superoxide dismutase (SOD), and glutathione (GSH). According to our results, rats treated with only 80% ethanol (vehicle group) exhibited significant increases in their ulcer area and ulcer index (UI). Moreover, the levels of ROS and MDA markedly increased in the vehicle group compared with the normal control group. Daily oral administration of Phy-Blica-D (500 and 1000 mg/kg) for 7 days not only significantly decreased the ulcer area and UI, but also remarkably decreased the ROS and MDA levels in gastric tissue. Gastric ulcers induced by ethanol had significantly decreased antioxidant enzyme activities (CAT and SOD) and non-enzymatic antioxidant (GSH), whereas pretreatment with Phy-Blica-D significantly improved the activities of CAT, SOD, and GSH. Moreover, after exposure to ethanol, the rats exhibited a significantly increased level of inducible nitric oxide synthase (iNOS), which was reduced after treatment with Phy-Blica-D. These findings suggest that Phy-Blica-D potentially exerts its gastroprotective effects by suppressing oxidative stress and stimulating antioxidant enzymes, which is one of the causes of destruction of cell membranes, and it is involved in the pathogenesis of acute gastric ulcers induced by ethanol. |
| Databáze: | OpenAIRE |
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