Cellular characteristics of long-term cultured rat parotid acinar cells
| Autor: | Bruce J. Baum, Prema M. Mertz, Chih Ko Yeh, Constance Oliver, Eleni E. Kousvelari |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Male
medicine.medical_specialty Time Factors Clinical Biochemistry Immunocytochemistry In situ hybridization Biology Cytoplasmic Granules Selenious Acid Antiviral Agents Selenium stomatognathic system Internal medicine medicine Protein biosynthesis Putrescine Animals Parotid Gland Secretion Dimethyl Sulfoxide RNA Messenger Cells Cultured Organelles Epidermal Growth Factor Isoproterenol Nucleic Acid Hybridization Cell Differentiation Rats Inbred Strains Cell Biology General Medicine Glutathione Immunohistochemistry Parotid gland Cell biology Rats medicine.anatomical_structure Endocrinology Cytoplasm Cell culture Amylases Proline-Rich Protein Domains Stem cell Peptides Cell Division Developmental Biology |
| Zdroj: | In vitro cellulardevelopmental biology : journal of the Tissue Culture Association. (9) |
| ISSN: | 0883-8364 |
| Popis: | We have successfully maintained and biochemically characterized differentiated rat parotid acinar cells cultured for long periods (6 mo.). The cells were cultured on a reconstituted basement membrane matrix in a medium containing a variety of agents that promote cellular proliferation and differentiation. The cultured cells retain the characteristics of the parental parotid acinar cells. They exhibit both secretory granules and abundant cellular organelles required for protein synthesis and secretion. In situ hybridization and immunocytochemistry demonstrate high levels of proline-rich protein mRNA and protein, and lower levels of amylase mRNA and protein, in their cytoplasm. These findings suggest that rat parotid acinar cells can be maintained in a differentiated state in vitro for long periods, and can serve as a useful model system for studying the regulation of exocrine secretory processes. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|