Nuclear localization of the CK2α-subunit correlates with poor prognosis in clear cell renal cell carcinoma

Autor: Aida Oliván-Viguera, Minne Line Nedergaard Mikkelsen, Niels Marcussen, Olaf-Georg Issinger, Barbara Guerra, Maj Rabjerg, Ralf Köhler
Rok vydání: 2016
Předmět:
RNA
Messenger/genetics
Male
0301 basic medicine
Pathology
0302 clinical medicine
Cell Movement
Renal cell carcinoma
Adenoma
Oxyphilic
Medicine
Nuclear protein
Casein Kinase II
Kidney Neoplasms/mortality
Aged
80 and over
Middle Aged
Kidney Neoplasms
Carcinoma
Renal Cell/mortality
CK2-targeted therapy
Renal cancer
Oncology
030220 oncology & carcinogenesis
Adenoma
Oxyphilic/mortality
CK2 subunits
Female
Biomarkers
Tumor/genetics
Casein kinase 2
Research Paper
Adult
medicine.medical_specialty
Naphthyridines/pharmacology
renal cancer
Cell Proliferation/genetics
03 medical and health sciences
Cell Line
Tumor
Cell Movement/genetics
Biomarkers
Tumor
Humans
Neoplasm Invasiveness
Protein kinase CK2
RNA
Messenger
Naphthyridines
Kinase activity
Protein kinase A
Carcinoma
Renal Cell
Aged
Cell Proliferation
business.industry
Cell growth
CX-4945
Cancer
medicine.disease
Clear cell renal cell carcinoma
030104 developmental biology
Casein Kinase II/antagonists & inhibitors
protein kinase CK2
Cancer research
Phenazines
Neoplasm Invasiveness/genetics
business
Zdroj: Zaguán. Repositorio Digital de la Universidad de Zaragoza
instname
Scopus-Elsevier
Oncotarget
Rabjerg, M, Guerra, B, Oliván-Viguera, A, Mikkelsen, M L N, Köhler, R, Issinger, O G & Marcussen, N 2017, ' Nuclear localization of the CK2α-subunit correlates with poor prognosis in Clear Cell Renal Cell Carcinoma ', Oncotarget, vol. 8, no. 1, pp. 1613-1627 . https://doi.org/10.18632/oncotarget.13693
ISSN: 1949-2553
Popis: // Maj Rabjerg 1 , Barbara Guerra 2 , Aida Olivan-Viguera 3 , Minne Line Nedergaard Mikkelsen 1 , Ralf Kohler 3 , Olaf-Georg Issinger 2 , Niels Marcussen 1 1 Department of Pathology, Odense University Hospital, DK-5000 Odense, Denmark 2 Department of Biochemistry and Molecular Biology, University of Southern Denmark, DK-5230 Odense, Denmark 3 Aragon Agency for Research and Development (ARAID), IACS, IIS Aragon, 50009 Zaragoza, Spain Correspondence to: Maj Rabjerg, email: mrabjerg@health.sdu.dk Keywords: renal cancer, protein kinase CK2, CK2 subunits, CK2-targeted therapy, CX-4945 Received: April 25, 2016 Accepted: November 11, 2016 Published: November 29, 2016 ABSTRACT Protein kinase CK2α, one of the two catalytic isoforms of the protein kinase CK2 has been shown to contribute to tumor development, tumor proliferation and suppression of apoptosis in various malignancies. We conducted this study to investigate CK2 expression in different subtypes of Renal Cell Carcinoma (RCC) and in the benign oncocytoma. qRT-PCR, immunohistochemistry and Western blot analyses revealed that CK2α expression was significantly increased at the mRNA and protein levels in clear cell RCC (ccRCC). Also the kinase activity of CK2 was significantly increased in ccRCC compared to normal renal cortex. Nuclear protein expression of CK2α correlated in univariate analysis with poor Progression Free Survival (HR = 8.11, p = 0.016). Functional analyses (cell proliferation assay) revealed an inhibitory effect of Caki-2 cell growth following CK2 inhibition with CX-4945. Our results suggest that CK2α promotes migration and invasion of ccRCC and therefore could serve as a novel prognostic biomarker and molecular therapeutic target in this type of cancer.
Databáze: OpenAIRE
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