Oligonucleotides--assembled Au nanorod-assisted cancer photothermal ablation and combination chemotherapy with targeted dual-drug delivery of Doxorubicin and Cisplatin prodrug
| Autor: | Yi-Hsin Chien, Chen-Sheng Yeh, Yu Sen Chen, Umesh Kumar, Chia Hao Su, Vijayakumar Shanmugam, Yu Sheng Cheng, Hua Fen Hsu, Tzu Yu Liu, Chih Chia Huang |
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| Rok vydání: | 2014 |
| Předmět: |
Male
Materials science Stereochemistry Oligonucleotides Mice Nude Antineoplastic Agents Mice Drug Delivery Systems In vivo Cell Line Tumor Neoplasms medicine Peptide bond Animals Humans General Materials Science Doxorubicin Prodrugs Cisplatin Drug Carriers Nanotubes Oligonucleotide Folate Receptors GPI-Anchored Combination chemotherapy Prodrug Phototherapy Combinatorial chemistry Drug delivery medicine.drug |
| Zdroj: | ACS applied materialsinterfaces. 6(6) |
| ISSN: | 1944-8252 |
| Popis: | External stimuli responsive dual drugs carrier was synthesized with Au nanorods (NRs) as the platform. On Au NRs, single stranded DNAs were assembled using 5' thiol end. Following this, complementary DNA (cDNA) strands were hybridized. This hybridized double stranded DNA facilitated doxorubicin (Dox) intercalation into the duplexes. The cDNA designed with the 5' amine functional group assisted to tether platinum [Pt(IV)] prodrugs by establishing amide bond with the acid group at the axial ligand. The other axial acid group in Pt(IV) prodrugs was conjugated with the folic acid (FA) to target folate receptors overexpressed in the cancer cells. This targeting vehicle provided remote-controlled delivery of this high toxic cargo cocktail at the tumor site, ensuring extra specificity that can avoid acute toxicity, where release of Dox and Pt(IV) was achieved upon NIR 808 nm diode laser irradiation. The dehybridization set the Dox free to bind the cell nucleus and cellular reductants reduced Pt(IV) to yield toxic Pt(II), becoming an active drug. The in vitro and in vivo studies revealed that this external stimulus responsive combination drug delivery was significantly effective. |
| Databáze: | OpenAIRE |
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