Extraction and analysis of free amino acids and 5′-nucleotides, the key contributors to the umami taste of seaweed
| Autor: | Klaas R. Timmermans, Geert Mol, Norbert Reuter, Jose M. de Winter, Roy Fremouw, Tanja C.W. Moerdijk-Poortvliet, Sandra de Reu, Goverdina C. H. Derksen, Dylan L. C. de Jong |
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| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
chemistry.chemical_classification
Taste biology Nucleotides Extraction (chemistry) Chlorophyceae Glutamic Acid General Medicine Glutamic acid Umami biology.organism_classification Seaweed High-performance liquid chromatography Analytical Chemistry Algae chemistry Nucleotide Food science Amino Acids Food Science |
| Popis: | Assessing the umami taste of seaweed on a chemical level can inform the use and selection of seaweed in European cuisine. Accordingly, we developed a method for the simultaneous extraction, separate clean-up and analysis of 21 free amino acids and 10 free nucleotides by reversed phase and mixed-mode HPLC respectively. Of multiple mouth emulating solvents, extracting in Milli-Q at 35 °C was found most suitable. This method showed good linearity (R2 > 0.9996), resolution (Rs ≥ 1.5) and picomole detection limits, and was successfully applied to determine the Equivalent Umami Concentration (EUC) and Taste Activity Values (TAV) of seven Dutch seaweed species. Phaeophyceae showedthe highest EUC, followed by Chlorophyceae and Rhodophyceae (≈ 9.5, 3.7 and 1.1 g/100 g respectively). Glutamic acid always exceeded the TAV, while other umami compounds were species specific. Our method can accurately predict umami intensity and therefore contributes towards species selection for the European palette. |
| Databáze: | OpenAIRE |
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