Gastroprotective mechanisms of the chloroform and ethyl acetate phases of Praxelis clematidea (Griseb.) R.M.KingH.Robinson (Asteraceae)
| Autor: | Heloina de Sousa Falcão, Christiane Takayama, Gabriela Lemos de Azevedo Maia, Clélia Akiko Hiruma Lima, Flavia Bonamin, Leônia Maria Batista, Anderson Luiz Ferreira, Thiago Mello Moraes, Hélio Kushima, Maria de Fátima Agra, José Maria Barbosa Filho, Alba Regina Monteiro Souza Brito |
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| Rok vydání: | 2011 |
| Předmět: |
Male
Glutathione reductase Drug Evaluation Preclinical Pharmacology Acetates Asteraceae Lipid peroxidation Superoxide dismutase chemistry.chemical_compound Mice Piroxicam Gastric mucosa medicine Animals Stomach Ulcer Rats Wistar chemistry.chemical_classification Plants Medicinal biology Ethanol Plant Extracts Glutathione peroxidase Glutathione Plant Components Aerial Malondialdehyde Anti-Ulcer Agents Rats Disease Models Animal Mucus medicine.anatomical_structure chemistry Cytoprotection Gastric Mucosa Myeloperoxidase Reperfusion Injury biology.protein Solvents Molecular Medicine Chloroform Biomarkers Phytotherapy Signal Transduction |
| Zdroj: | Journal of natural medicines. 67(3) |
| ISSN: | 1861-0293 |
| Popis: | Flavonoid-rich Praxelis clematidea (Griseb.) R.M.King & H.Robinson (Asteraceae) is a native plant of South America. This study evaluates the gastroprotective activity and possible mechanisms for both the chloroform (CHCl3P) and ethyl acetate phases (AcOEtP) obtained from aerial parts of the plant. The activity was investigated using acute models of gastric ulcer. Gastric secretion biochemical parameters were determined after pylorus ligature. The participation of cytoprotective factors such as mucus, nitric oxide (NO), sulfhydryl (SH) groups, prostaglandin E2 (PGE2), reduced glutathione (GSH), superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione reductase (GR), reduction of lipid peroxidation (malondialdehyde level), and polymorphonuclear infiltration (myeloperoxidase activity), was also investigated. CHCl3P (125, 250, and 500 mg/kg) and AcOEtP (62.5, 125, and 250 mg/kg) showed significant gastroprotective activity, reducing the ulcerative index by 75, 83, 88 % and 66, 66, 81 % for ethanol; 67, 67, 56 % and 56, 53, 58 % for a non-steroidal anti-inflammatory drug (NSAID); and 74, 58, 59 % and 64, 65, 61 % for stress-induced gastric ulcer, respectively. CHCl3P (125 mg/kg) and AcOEtP (62.5 mg/kg) significantly reduced the ulcerative area by 78 and 83 %, respectively, for the ischemia–reperfusion model. They also did not alter the biochemical parameters of gastric secretion, the GSH level or the activities of SOD, GPx or GR. They increased the quantity of gastric mucus, not dependent on NO, yet dependent on SH groups, and maintained PGE2 levels. The P. clematidea phases demonstrated gastroprotective activity related to cytoprotective factors. |
| Databáze: | OpenAIRE |
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