Diabetes alters inflammation, angiogenesis, and fibrogenesis in intraperitoneal implants in rats
| Autor: | Anilton Cesar Vasconcelos, Paula Peixoto Campos, Irma X. Barbosa, Núbia Braga Pereira, Silvia Passos Andrade, Teresa Oviedo-Socarrás |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Male
Surgical Sponges Vascular Endothelial Growth Factor A Pathology medicine.medical_specialty Time Factors Angiogenesis Polyurethanes Inflammation Biochemistry Diabetes Mellitus Experimental Neovascularization Transforming Growth Factor beta1 Hemoglobins Fibrosis Diabetes mellitus medicine Animals Rats Wistar Chemokine CCL2 Wound Healing Neovascularization Pathologic business.industry Tumor Necrosis Factor-alpha Foreign-Body Reaction Cell Biology medicine.disease Streptozotocin Platelet Endothelial Cell Adhesion Molecule-1 Immunology Implant Collagen medicine.symptom Inflammation Mediators Cardiology and Cardiovascular Medicine business Wound healing medicine.drug Ethers |
| Zdroj: | Microvascular research. 93 |
| ISSN: | 1095-9319 |
| Popis: | The increased prevalence of diabetes worldwide is associated with increasing numbers of diabetic individuals receiving synthetic matrices and biomedical implants to repair and/or replace biological tissues. This therapeutic procedure invariably leads to adverse tissue healing (foreign body reaction), thus impairing the biomedical device function of subcutaneous implants. However, the influence of diabetes on abnormal tissue healing in intraperitoneal implants is unclear. We investigated key components of foreign body reactions in diabetic rats. Polyether-polyurethane sponge discs were placed intraperitoneally in rats previously injected with streptozotocin for induction of diabetes and in non-diabetic rats. Implants removed 10 days after implantation were assessed by determining the components of the fibrovascular tissue (angiogenesis, inflammation, and fibrogenesis). In implants from diabetic rats, fibrous capsule thickness and fibrovascular tissue infiltration (hematoxylineosin and picrosirius staining) were reduced in comparison with implants from non-diabetic rats. Hemoglobin (Hb) content (vascular index) and VEGF levels (pro-angiogenic cytokine) were increased after diabetes. However, the number of vessels (HE and CD31-immunostaining) in the fibrovascular tissue from diabetic rats was decreased when compared with vessel numbers in implants from non-diabetic animals. Overall, all inflammatory parameters (macrophage accumulation-NAG activity; TNF-α and MCP-1 levels) increased in intraperitoneal implants after diabetes induction. The pro-fibrogenic cytokine (TGFβ-1) increased after diabetes, but collagen deposition remained unaltered in the implants from diabetic rats. These important diabetes-related changes (increased levels of pro-inflammatory and angiogenic and fibrogenic cytokines) in peritoneal implant healing provide an insight into the mechanisms of the foreign body response in the diabetic environment in rats. |
| Databáze: | OpenAIRE |
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