Effect of Immobilisation on the Production of α-Amylase by an Industrial Strain of Bacillus amyloliquefaciens
| Autor: | K. Thayanithy, George Argirakos, D. A. John Wase |
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| Rok vydání: | 2007 |
| Předmět: |
Time Factors
Calcium alginate Bacillus amyloliquefaciens Alginates General Chemical Engineering Bacillus Industrial fermentation Inorganic Chemistry chemistry.chemical_compound Glucuronic Acid Bioreactor Amylase Lactose Waste Management and Disposal Bacteriological Techniques Chromatography biology Renewable Energy Sustainability and the Environment Hexuronic Acids Organic Chemistry Glucuronic acid biology.organism_classification Pollution Kinetics Fuel Technology chemistry Biochemistry biology.protein Fermentation alpha-Amylases Biotechnology |
| Zdroj: | Journal of Chemical Technology & Biotechnology. 53:33-38 |
| ISSN: | 1097-4660 0268-2575 |
| DOI: | 10.1002/jctb.280530106 |
| Popis: | The effect of immobilisation of an industrial strain of Bacillus amyloliquefaciens in calcium alginate beads on production of alpha-amylase was investigated using lactose-based media in shake flasks and in a 0.3 dm3 glass fermenter. Although the microorganism was a good alpha-amylase producer in batch cultures of free cells, it was unable to produce the enzyme for extended periods either in repeated batch cultures, or in continuous cultivation. In each case, parallel tests with cells immobilised in calcium alginate beads gave still further reduced enzyme yields, and the free cells released into the broth from these beads probably contributed substantially to any amylase produced during these extended fermentations. After prolonged use, the core of the alginate beads accumulated hard insoluble material, with viable immobilised cells confined to a surface layer. |
| Databáze: | OpenAIRE |
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