Fructose-Rich Diet Attenuates Stress-Induced Metabolic Disturbances in the Liver of Adult Female Rats
| Autor: | Ana Djordjevic, Gordana Matić, Jelena Brkljačić, Luc Tappy, Ivana Elaković, Danijela Vojnović Milutinović, Duško Blagojević, Sanja Kovačević, Aleksandra Nikolić-Kokić |
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| Rok vydání: | 2021 |
| Předmět: |
medicine.medical_specialty
Medicine (miscellaneous) 030209 endocrinology & metabolism Fructose Carbohydrate metabolism medicine.disease_cause 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Internal medicine medicine Animals Chronic stress Rats Wistar Beta oxidation 030304 developmental biology 0303 health sciences Nutrition and Dietetics Lipogenesis AMPK Diet Rats Endocrinology Liver chemistry Gluconeogenesis Female Oxidative stress |
| Zdroj: | The Journal of Nutrition. 151:3661-3670 |
| ISSN: | 0022-3166 |
| DOI: | 10.1093/jn/nxab294 |
| Popis: | BACKGROUND Both fructose consumption and chronic stress contribute to the development of metabolic disorders. The consequences of such combination are not fully understood. OBJECTIVE We investigated whether fructose supplementation and chronic stress synergistically disturb hepatic lipid and glucose metabolism. The role of energy sensing, redox, and inflammatory status during development of metabolic disturbances was investigated. METHODS Female Wistar rats, aged 2.5 mo, were divided into 4 experimental groups: control (C) fed a standard diet (commercial food and drinking water); fructose (F) fed the same food and 10% fructose solution; stress (S) fed the standard diet and subjected to chronic unpredictable stress and, stress + fructose (SF) combining conditions F and S as above. Stress included daily stressors: cold water forced swimming, physical restraint, cold room, wet bedding, rocking, switching, or tilting cages. After 9 wk, hepatic enzymes and transcription factors involved in gluconeogenesis, lipogenesis, fatty acid oxidation, antioxidative defence, energy sensing, and cytokines were assessed by qPCR, Western blotting, and spectrophotometry and analyzed by 2-factor ANOVA. RESULTS Fructose increased AMP-activated protein kinase (AMPK) phosphorylation (40%; P |
| Databáze: | OpenAIRE |
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