Correction to: MicroRNA‑214 promotes alveolarization in neonatal rat models of bronchopulmonary dysplasia via the PlGF‑dependent STAT3 pathway
| Autor: | Xian‑Mei Huang, Hui Hong, Xiao‑Xia Li, Zhi‑Qun Zhang, Jing Li |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Hyperoxia
Lung business.industry RM1-950 QD415-436 medicine.disease Molecular medicine Biochemistry Gene expression profiling medicine.anatomical_structure Bronchopulmonary dysplasia mental disorders microRNA Survivin Genetics medicine Cancer research Molecular Medicine Gene silencing Therapeutics. Pharmacology medicine.symptom business Molecular Biology Genetics (clinical) |
| Zdroj: | Molecular Medicine, Vol 27, Iss 1, Pp 1-3 (2021) |
| ISSN: | 1528-3658 1076-1551 |
| Popis: | BACKGROUND Recently, the role of several microRNAs (miRNAs or miRs) in pulmonary diseases has been described. The molecular mechanisms by which miR-214 is possibly implicated in bronchopulmonary dysplasia (BPD) have not yet been addressed. Hence, this study aimed to investigate a putative role of miR-214 in alveolarization among preterm neonates with BPD. METHODS Microarray-based gene expression profiling data from BPD was employed to identify differentially expressed genes. A BPD neonatal rat model was induced by hyperoxia. Pulmonary epithelial cells were isolated from rats and exposed to hyperoxia to establish cell injury models. Gain- and loss-of-function experiments were performed in BPD neonatal rats and hyperoxic pulmonary epithelial cells. MiR-214 and PlGF expression in BPD neonatal rats, and eNOS, Bcl-2, c-myc, Survivin, α-SMA and E-cadherin expression in hyperoxic pulmonary epithelial cells were measured using RT-qPCR and Western blot analysis. The interaction between PlGF and miR-214 was identified using dual luciferase reporter gene and RIP assays. IL-1β, TNF-a, IL-6, ICAM-1 and Flt-1 expression in the rat models was measured using ELISA. RESULTS The lung tissues of neonatal rats with BPD showed decreased miR-214 expression with elevated PlGF expression. PlGF was found to be a target of miR-214, whereby miR-214 downregulated PlGF to inactivate the STAT3 pathway. miR-214 overexpression or PlGF silencing decreased the apoptosis of hyperoxic pulmonary epithelial cells in vitro and restored alveolarization in BPD neonatal rats. CONCLUSION Overall, the results demonstrated that miR-214 could facilitate alveolarization in preterm neonates with BPD by suppressing the PlGF-dependent STAT3 pathway. |
| Databáze: | OpenAIRE |
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