Calpain-mediated regulation of stargazin in adult rat brain
| Autor: | Karoline Rostamiani, Yu-Tien Hsu, Lifei Yu, Xiaoning Bi, Yi Wang, Michel Baudry |
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| Rok vydání: | 2011 |
| Předmět: |
Male
medicine.medical_specialty Central nervous system Immunocytochemistry Hippocampus AMPA receptor In Vitro Techniques Article Rats Sprague-Dawley Internal medicine medicine Neuropil Animals biology Calpain General Neuroscience Glutamate receptor Brain Membrane Proteins Dipeptides Transmembrane protein Rats Cell biology medicine.anatomical_structure Endocrinology nervous system biology.protein Calcium Calcium Channels |
| Zdroj: | Neuroscience. 178:13-20 |
| ISSN: | 0306-4522 |
| DOI: | 10.1016/j.neuroscience.2011.01.026 |
| Popis: | Changes in AMPA receptors have been proposed to underlie changes in synaptic efficacy in hippocampus and other brain structures. Calpain activation has also been discussed as a potential mechanism to produce lasting modifications of synaptic structure and function. Stargazin is a member of the family of transmembrane AMPA receptor associated proteins (TARPs), which participate in trafficking of AMPA receptors and regulate their kinetic properties. We report here that preincubation of thin (20 microns) frozen rat brain sections with calcium changes the immunological properties of stargazin, an effect totally blocked by a calpain inhibitor. Immunocytochemistry indicates that in situ calpain activation produces a decreased immunoreactivity for stargazin in the neuropil throughout the brain, and western blots confirmed that a similar treatment decreased stargazin levels. Interestingly, the same treatment did not modify the immunoreactivity for another TARP member, γ-8, although it increased immunoreactivity in cell bodies in hippocampus, an effect that was not blocked by calpain inhibition. These results strongly suggest the involvement of calpain in the regulation of AMPA receptor targeting and function through truncation of stargazin. |
| Databáze: | OpenAIRE |
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