Discovery of frequent homozygous deletions in chromosome 3p21.3 LUCA and AP20 regions in renal, lung and breast carcinomas
| Autor: | John D. Minna, Debora Angeloni, Jian Liu, Vladimir I. Kashuba, Eleonora A. Braga, Lev L. Kisselev, Witaly Loginov, Eugene R. Zabarovsky, V. N. Senchenko, Yury Seryogin, R. F. Garkavtseva, Veronika I. Zabarovska, Tatiana P. Kazubskaya, Michael I. Lerman, V. D. Ermilova, Igor Bazov, George Klein |
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| Rok vydání: | 2004 |
| Předmět: |
Genetic Markers
Cancer Research Lung Neoplasms ITGA9 Tumor suppressor gene Loss of Heterozygosity Breast Neoplasms Nerve Tissue Proteins Semaphorins Biology medicine.disease_cause Polymerase Chain Reaction Loss of heterozygosity Tumor Cells Cultured Genetics medicine Humans Carcinoma Small Cell Lung cancer Carcinoma Renal Cell Molecular Biology Sequence Deletion Chromosome Aberrations Gene Rearrangement Membrane Glycoproteins Carcinoma Homozygote Membrane Proteins Chromosome Gene rearrangement medicine.disease Molecular biology Kidney Neoplasms Genetic marker Female Calcium Channels Chromosomes Human Pair 3 Carcinogenesis |
| Zdroj: | Oncogene. 23:5719-5728 |
| ISSN: | 1476-5594 0950-9232 |
| DOI: | 10.1038/sj.onc.1207760 |
| Popis: | We searched for chromosome 3p homo- and hemizygous losses in 23 lung cancer cell lines, 53 renal cell and 22 breast carcinoma biopsies using 31 microsatellite markers located in frequently deleted 3p regions. In addition, two sequence-tagged site markers (NLJ-003 and NL3-001) located in the Alu-PCR clone 20 region (AP20) and lung cancer (LUCA) regions, respectively, were used for quantitative real-time PCR (QPCR). We found frequent (10-18%) homozygous deletions (HDs) in both 3p21.3 regions in the biopsies and lung cancer cell lines. In addition, we discovered that amplification of 3p is a very common (15-42.5%) event in these cancers and probably in other epithelial malignancies. QPCR showed that aberrations of either NLJ-003 or NL3-001 were detected in more than 90% of all studied cases. HDs were frequently detected simultaneously both in NLJ-003 or NL3-001 loci in the same tumour (P |
| Databáze: | OpenAIRE |
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