Cloning and expression of 130-kd mosquito-larvicidal δ-endotoxin gene of Bacillus thuringiensis var. Israelensis in Escherichia coli
Autor: | Sunee Kertbundit, Chatri Settasatian, Sakol Panyim, Wipa Chungjatupornchai, Plernpis Luxananil, Chanan Angsuthanasombat, Prapon Wilairat |
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Rok vydání: | 1987 |
Předmět: |
Insecticides
Transcription Genetic Bacterial Toxins Bacillus thuringiensis Aedes aegypti Molecular cloning medicine.disease_cause law.invention Microbiology Hemolysin Proteins Plasmid Bacterial Proteins law Escherichia coli Genetics medicine Amino Acid Sequence Cloning Molecular Molecular Biology Gene Bacillus thuringiensis Toxins Base Sequence biology Nucleic acid sequence DNA Restriction Enzymes Plants biology.organism_classification Molecular biology Endotoxins Culicidae Genes Genes Bacterial Recombinant DNA |
Zdroj: | Molecular and General Genetics MGG. 208:384-389 |
ISSN: | 1432-1874 0026-8925 |
Popis: | Five recombinant E. coli clones exhibiting toxicity to Aedes aegypti larvae were obtained from a library of 800 clones containing XbaI DNA fragments of 110 kb plasmid from B. thuringiensis var. israelensis. All the five clones (pMU 14/258/303/388/679) had the same 3.8-kb insert and encoded a major protein of 130 kDa which was highly toxic to A. aegypti larvae. Three clones (pMU 258/303/388) transcribed the 130 kD a gene in the same direction as that of lac Z promoter of pUC12 vector whereas the transcription of the other two (pMU 14/679) was in the opposite direction. A 1.9-kb fragment of the 3.8 kb insert coded for a protein of 65 kDa. Partial DNA sequence of the 3.8 kb insert, corresponding to the 5'-terminal of the 130 kDa gene, revealed a continuous reading frame, a Shine-Dalgarno sequence and a tentative 5'-regulatory region. These results demonstrated that the 3.8 kb insert is a minimal DNA fragment containing a regulatory region plus the coding sequence of the 130 kDa protein that is highly toxic to mosquito larvae. |
Databáze: | OpenAIRE |
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