Coccidial contamination of raspberries: mock contamination with Eimeria acervulina as a model for decontamination treatment studies
| Autor: | Eng-Hong Lee, Marilyn B. Lee |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Veterinary medicine
Hot Temperature Dose-Response Relationship Radiation Human decontamination Biology Contamination biology.organism_classification Microbiology Cyclospora Eimeria acervulina Disinfection Feces Gamma Rays Treatment study Fruit Freezing Oocytes Animals Eimeria Cecum Chickens Food Science Gamma irradiation |
| Zdroj: | Journal of food protection. 64(11) |
| ISSN: | 0362-028X |
| Popis: | Numerous outbreaks have been reported since 1995 in the United States and Canada that were linked to the consumption of imported fresh raspberries contaminated with Cyclospora. Because Cyclospora has no laboratory animal hosts, Eimeria acervulina, a common chicken coccidium similar in characteristics to Cyclospora, was used as a surrogate to test decontamination treatments. Raspberries were mock contaminated with E. acervulina-sporulated oocysts in a water suspension, then exposed to washing, freezing, heat, or irradiation before they were fed to chicks. The presence of oocysts in the contaminated raspberries was confirmed either by duodenal lesions or oocysts in cecal contents 5 days postinoculation (PI) or in fecal contents 6 days PI, after 24 h of fecal collection. Washing of raspberries was generally not adequate in removing coccidial contamination, but freezing and heat treatment appeared effective. Gamma irradiation of E. acervulina-sporulated oocysts at a dose of 0.5 kGy was partially effective, but it was completely effective at 1.0 kGy and higher. We suggest that E. acervulina, for mock contamination of raspberries and subsequent decontamination treatments, is easy to handle, safe, and economical to study. |
| Databáze: | OpenAIRE |
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