Marker of coxsackievirus-B4 infection in saliva of patients with type 1 diabetes
| Autor: | Famara Sane, C Lefevre, J Weill, Monzer Hamze, Francis Badia-Boungou, Juliano G. Haddad, I Gueorguieva, P A Opoko, C Stukens, Henry Germain Monabeka, Donatien Moukassa, Enagnon Kazali Alidjinou, Mohamad Bachar Ismail, Ange Antoine Abena, E Bouenizabila, Matthieu Ternois, Didier Hober |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Adult Male Saliva Adolescent Endocrinology Diabetes and Metabolism Coxsackievirus Infections Coxsackievirus medicine.disease_cause Pathogenesis 03 medical and health sciences Young Adult Endocrinology Diabetes mellitus Internal Medicine medicine Humans Child Cytopathic effect Type 1 diabetes biology business.industry Infant medicine.disease biology.organism_classification Antibodies Neutralizing Enterovirus B Human 030104 developmental biology Diabetes Mellitus Type 1 Child Preschool Coxsackieviruses B Immunology Enterovirus Female business |
| Zdroj: | Diabetes/metabolism research and reviews. 33(7) |
| ISSN: | 1520-7560 |
| Popis: | Background Coxsackieviruses B (CV-B) are enteroviruses that have been reported to play a role in the pathogenesis of type 1 diabetes. Enteroviral RNA was detected in the gut mucosa of patients. The mucosal immunity is an interconnected network; therefore, the response to enteroviruses possibly present in the gastrointestinal mucosa can be reflected by specific antibodies in the saliva. In the present study, the anti-CV-B neutralizing activity of saliva samples from patients with type 1 diabetes was investigated. Methods Saliva samples were collected from patients and controls of 3 countries, and plasma was obtained from some of them. The anti-CV-B activity of clinical samples was determined by neutralization of the cytopathic effect induced by challenging viruses in vitro and expressed as titre value. Results Overall prevalence and levels of anti-CV-B4 activity of saliva were higher in patients (n = 181) than in controls (n = 135; P = .0002; titre values ≥ 16: odds ratio = 4.22 95% CI: 1.90-9.38 P = .0002). It has been shown that IgA1 played a role in this activity. There was no correlation between the saliva and the plasma anti-CV-B4 neutralizing activity. The neutralizing activity of saliva against CV-B1, CV-B2, CV-B3, and CV-B5 existed rarely, if at all. Increased levels of anti-CV-B4 activity were observed all along a 4 year follow-up period in patients but not in matched controls (P = .01). Conclusion There is an anti-CV-B4 activity in saliva of patients with type 1 diabetes that may be a useful marker to study the role of CV-B in the pathogenesis of the disease. |
| Databáze: | OpenAIRE |
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