Local immune response and protection in the guinea pig keratoconjunctivitis model following immunization with Shigella vaccines
| Autor: | David N. Taylor, Antoinette B. Hartman, C Powell, L L Van De Verg, N O Bendiuk, D B Tang, H H Collins |
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| Rok vydání: | 1994 |
| Předmět: |
Keratoconjunctivitis
Infectious animal diseases Injections Subcutaneous Immunology Guinea Pigs Immunization Secondary chemical and pharmacologic phenomena Biology Vaccines Attenuated Microbiology Shigella flexneri Blood serum Immune system Conjugate vaccine Immunity Animals Shigella vaccine Antibody-Producing Cells Dysentery Bacillary Vaccines Synthetic Attenuated vaccine Mucous Membrane Polysaccharides Bacterial O Antigens biochemical phenomena metabolism and nutrition Virology Antibodies Bacterial Immunoglobulin A Bacterial vaccine Disease Models Animal Infectious Diseases Bacterial Vaccines biology.protein bacteria Parasitology Lymph Nodes Antibody Injections Intraperitoneal Spleen Research Article |
| Zdroj: | Infection and immunity. 62(2) |
| ISSN: | 0019-9567 |
| Popis: | This study used the guinea pig keratoconjunctivitis model to examine the importance of route of administration (mucosal versus parenteral), frequency and timing of immunization (primary versus boosting immunization), and form of antigen given (live attenuated vaccine strain versus O-antigen-protein conjugate) on the production of protective immunity against Shigella infection. Since local immune response to the lipopolysaccharide (LPS) O-antigen of Shigella spp. is thought to be important for protection against disease, O-antigen-specific antibody-secreting cells (ASC) in the spleen and regional lymph nodes of immunized animals were measured by using an ELISPOT assay. Results indicated that protective efficacy was associated with a strong O-antigen-specific ASC response, particularly in the superficial ventral cervical lymph nodes draining the conjunctivae. In naive animals, a strong ASC response in the cervical lymph nodes and protection against challenge were detected only in animals that received a mucosal immunization. Protection in these animals was increased by a boosting mucosal immunization. While parenteral immunization alone with an O-antigen-protein conjugate vaccine did not protect naive animals against challenge, a combined parenteral-mucosal regimen elicited enhanced protection without the addition of a boosting immunization. Although O-antigen-specific serum immunoglobulin A titers were significantly higher in animals receiving a mucosal immunization, there was no apparent correlation between levels of serum antibody and protection against disease. |
| Databáze: | OpenAIRE |
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