Independent evolution of functionally exchangeable mitochondrial outer membrane import complexes
| Autor: | Daniela G Vitali, Sandro Käser, Antonia Kolb, Kai S Dimmer, Andre Schneider, Doron Rapaport |
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| Rok vydání: | 2017 |
| Předmět: |
Saccharomyces cerevisiae Proteins
QH301-705.5 Science Trypanosoma brucei brucei Protozoan Proteins Saccharomyces cerevisiae outer membrane MIM complex Mitochondrial Membrane Transport Proteins Biological Coevolution trypanosome Gene Expression Regulation Fungal 540 Chemistry Protein Isoforms Biology (General) Phosphorylation Organelle Biogenesis Genetic Complementation Test Membrane Proteins biogenesis Mitochondria Mitochondrial Membranes 570 Life sciences biology Medicine Gene Deletion |
| Zdroj: | eLife, Vol 7 (2018) Vitali, Daniela G; Käser, Sandro; Kolb, Antonia; Dimmer, Kai S; Schneider, André; Rapaport, Doron (2018). Independent evolution of functionally exchangeable mitochondrial outer membrane import complexes. eLife, 7 eLife Sciences Publications 10.7554/eLife.34488 |
| ISSN: | 2050-084X |
| DOI: | 10.7554/eLife.34488 |
| Popis: | Assembly and/or insertion of a subset of mitochondrial outer membrane (MOM) proteins, including subunits of the main MOM translocase, require the fungi-specific Mim1/Mim2 complex. So far it was unclear which proteins accomplish this task in other eukaryotes. Here, we show by reciprocal complementation that the MOM protein pATOM36 of trypanosomes is a functional analogue of yeast Mim1/Mim2 complex, even though these proteins show neither sequence nor topological similarity. Expression of pATOM36 rescues almost all growth, mitochondrial biogenesis, and morphology defects in yeast cells lacking Mim1 and/or Mim2. Conversely, co-expression of Mim1 and Mim2 restores the assembly and/or insertion defects of MOM proteins in trypanosomes ablated for pATOM36. Mim1/Mim2 and pATOM36 form native-like complexes when heterologously expressed, indicating that additional proteins are not part of these structures. Our findings indicate that Mim1/Mim2 and pATOM36 are the products of convergent evolution and arose only after the ancestors of fungi and trypanosomatids diverged. |
| Databáze: | OpenAIRE |
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