The evolution and genetic diversity of avian influenza A(H9N2) viruses in Cambodia, 2015 – 2016
| Autor: | Philippe Dussart, Ian G. Barr, Matthew Kaye, Songha Tok, Sothyra Tum, Ponnarath Keo, Erik A. Karlsson, Sokhoun Yann, Dhanasekaran Vijaykrishna, San Sorn, Aeron C. Hurt, Yi Mo Deng, Paul F. Horwood, Andrew R. Greenhill, Davun Holl, Annika Suttie, Merryn Roe, Srey Viseth Horm |
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| Přispěvatelé: | Unité de Virologie / Virology Unit [Phnom Penh], Institut Pasteur du Cambodge, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Federation University [Churchill, Australia], The Peter Doherty Institute for Infection and Immunity [Melbourne], University of Melbourne-The Royal Melbourne Hospital, Ministry of Agriculture, Forestry and Fisheries [Cambodia], Monash University [Melbourne], James Cook University (JCU), This publication is the result of work conducted under a cooperative agreement with the Office of the Assistant Secretary for Preparedness and Response in the U.S. Department of Health and Human Services (HHS), grant number IDSEP140020-01-00 (PH). The study was also funded, in part, by the US Agency for International Development (grant No. AID-442-G-14-00005) (PH). The Melbourne WHO Collaborating Centre for Reference and Research on Influenza is supported by the Australian Government Department of Health (IB)., The authors would like to thank the field team from the National Animal Health and Production Research Institute (Cambodian Ministry of Agriculture, Forestry and Fisheries), and the field and laboratory teams from the Virology Unit at the Pasteur Institute in Cambodia. |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
animal diseases viruses Reassortment Pathology and Laboratory Medicine Bird Genomics medicine.disease_cause [SDV.BID.SPT]Life Sciences [q-bio]/Biodiversity/Systematics Phylogenetics and taxonomy Poultry MESH: Poultry Zoonoses Genotype Medicine and Health Sciences Influenza A Virus H9N2 Subtype Influenza A virus MESH: Animals MESH: Genetic Variation MESH: Phylogeny Phylogeny MESH: Evolution Molecular Data Management Viral Genomics Multidisciplinary biology Neuraminidase inhibitor Eukaryota virus diseases Phylogenetic Analysis Genomics Phylogenetics Infectious Diseases Medical Microbiology Viral Pathogens Vertebrates Viruses [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology Medicine Pathogens MESH: Genome Viral Cambodia Research Article Computer and Information Sciences medicine.drug_class Science 030106 microbiology education Hemagglutinin (influenza) Microbial Genomics Genome Viral Microbiology Birds Evolution Molecular 03 medical and health sciences MESH: Influenza in Birds Microbial Control Virology Genetics medicine Animals Evolutionary Systematics Microbial Pathogens Taxonomy Pharmacology Evolutionary Biology Genetic diversity MESH: Cambodia Organisms Biology and Life Sciences Genetic Variation Influenza A virus subtype H5N1 MESH: Influenza A Virus H9N2 Subtype 030104 developmental biology Animal Genomics Influenza in Birds Amniotes biology.protein Antimicrobial Resistance |
| Zdroj: | PLoS ONE PLoS ONE, Public Library of Science, 2019, 14 (12), pp.e0225428. ⟨10.1371/journal.pone.0225428⟩ PLoS ONE, Vol 14, Iss 12, p e0225428 (2019) |
| ISSN: | 1932-6203 |
| Popis: | International audience; Low pathogenic A(H9N2) subtype avian influenza viruses (AIVs) were originally detected in Cambodian poultry in 2013, and now circulate endemically. We sequenced and characterised 64 A(H9N2) AIVs detected in Cambodian poultry (chickens and ducks) from January 2015 to May 2016. All A(H9) viruses collected in 2015 and 2016 belonged to a new BJ/94like h9-4.2.5 sub-lineage that emerged in the region during or after 2013, and was distinct to previously detected Cambodian viruses. Overall, there was a reduction of genetic diversity of H9N2 since 2013, however two genotypes were detected in circulation, P and V, with extensive reassortment between the viruses. Phylogenetic analysis showed a close relationship between A(H9N2) AIVs detected in Cambodian and Vietnamese poultry, highlighting cross-border trade/movement of live, domestic poultry between the countries. Wild birds may also play a role in A(H9N2) transmission in the region. Some genes of the Cambodian isolates frequently clustered with zoonotic A(H7N9), A(H9N2) and A(H10N8) viruses, suggesting a common ecology. Molecular analysis showed 100% of viruses contained the hemagglutinin (HA) Q226L substitution, which favours mammalian receptor type binding. All viruses were susceptible to the neuraminidase inhibitor antivirals; however, 41% contained the matrix (M2) S31N substitution associated with resistance to adamantanes. Overall, Cambodian A(H9N2) viruses possessed factors known to increase zoonotic potential, and therefore their evolution should be continually monitored. |
| Databáze: | OpenAIRE |
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