Investigation of architecture development and phosphate distribution in Chlorella biofilm by complementary microscopy techniques
| Autor: | Luigi Frunzo, Antonino Pollio, Mechthild Schmitt-Jansen, Hryhoriy Stryhanyuk, Hans H. Richnow, Jairo Hernán Moreno Osorio, Matthias Schmidt, Piet N.L. Lens, Pietro Benettoni, Niculina Musat, Gabriele Pinto, Giovanni Esposito |
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| Přispěvatelé: | Osorio, Jairo H Moreno, Benettoni, Pietro, Schmidt, Matthia, Stryhanyuk, Hryhoriy, Schmitt-Jansen, Mechthild, Pinto, Gabriele, Pollio, Antonino, Frunzo, Luigi, Lens, Piet N L, Richnow, Hans H, Esposito, Giovanni, Musat, Niculina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
030106 microbiology Chlorella vulgaris Spectrometry Mass Secondary Ion chemistry.chemical_element multimodal imaging Chlorella Biology Waste Disposal Fluid Applied Microbiology and Biotechnology Microbiology Phosphates Industrial wastewater treatment 03 medical and health sciences chemistry.chemical_compound Chlorella specie Microalgae Chlorella pyrenoidosa chemical imaging Microscopy biofilm development Ecology Phosphorus HIM Biofilm biochemical phenomena metabolism and nutrition biology.organism_classification Phosphate 030104 developmental biology chemistry Biofilms Environmental chemistry Green algae ToF-SIMS phosphorous distribution |
| Zdroj: | FEMS Microbiology Ecology. 95 |
| ISSN: | 1574-6941 |
| DOI: | 10.1093/femsec/fiz029 |
| Popis: | Microalgae biofilms may play an important role in the mitigation and prevention of eutrophication caused by domestic, agricultural and industrial wastewater effluents. Despite their potential, the biofilm development and role in nutrient removal are not well understood. Its clarification requires comprehensive studies of the complex three-dimensional architecture of the biofilm. In this study, we established a multimodal imaging approach to provide key information regarding architecture development and nutrient distribution in the biofilm of two green algae organisms: Chlorella pyrenoidosa and Chlorella vulgaris. Helium ion microscopy (HIM), scanning electron microscopy coupled with energy dispersive X-ray analysis (SEM-EDX) and time-of-flight secondary ion mass spectrometry (ToF-SIMS) were employed for i) elucidation of spatial arrangement, ii) elemental mapping and iii) 3D chemical imaging of the biofilm. The fine structure of the algal biofilm was resolved by HIM, the evidence of the accumulation of phosphate in hot spots was provided by SEM-EDX and the localization of phosphate oxides granules throughout the whole sample was clarified by ToF-SIMS. The reported results shed light on the phosphorus distribution during Chlorella's biofilm formation and highlight the potential of such correlative approach to solve fundamental question in algal biotechnology research. |
| Databáze: | OpenAIRE |
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