Bilateral Changes After Neonatal Ischemia in the P7 Rat Brain
| Autor: | Jean Mariani, Sonia Villapol, Valérie Biran, Catherine Goyenvalle, Maria Spiegler, Christiane Charriaut-Marlangue, Sylvain Renolleau |
|---|---|
| Přispěvatelé: | Neurobiologie des processus adaptatifs (NPA), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS), CHU Charles Foix [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), GIS, Institut de la longevite et du vieillissement, CHU Trousseau [APHP], Service de réanimation néonatale et pédiatrique [CHU Trousseau], Université Pierre et Marie Curie - Paris 6 (UPMC)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-CHU Trousseau [APHP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Sorbonne Université (SU) |
| Rok vydání: | 2007 |
| Předmět: |
Doublecortin Domain Proteins
Male MESH: Cell Death MESH: Nerve Regeneration Pathology Time Factors [SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology MESH: Neurons Fluorescent Antibody Technique MESH: Neuropeptides MESH: Animals Newborn Brain Ischemia Subgranular zone Brain ischemia 0302 clinical medicine MESH: Animals MESH: Fluorescent Antibody Technique Neurons 0303 health sciences Cell Death biology Neurogenesis Brain MESH: Brain Ischemia MESH: Oligodendroglia General Medicine Anatomy Immunohistochemistry Oligodendroglia medicine.anatomical_structure Neurology Female medicine.symptom Microtubule-Associated Proteins MESH: Ki-67 Antigen medicine.medical_specialty Doublecortin Protein MESH: Rats Ischemia Subventricular zone Pathology and Forensic Medicine Lesion MESH: Brain 03 medical and health sciences Cellular and Molecular Neuroscience MESH: Cell Proliferation medicine Animals Cell Proliferation 030304 developmental biology business.industry Neuropeptides MESH: Time Factors MESH: Immunohistochemistry medicine.disease MESH: Male Nerve Regeneration Rats Doublecortin MESH: Microtubule-Associated Proteins Ki-67 Antigen Animals Newborn nervous system biology.protein Neurology (clinical) business MESH: Female 030217 neurology & neurosurgery Immunostaining |
| Zdroj: | Journal of Neuropathology and Experimental Neurology Journal of Neuropathology and Experimental Neurology, 2007, 66 (6), pp.481-90. ⟨10.1097/01.jnen.0000263875.22306.3c⟩ Journal of Neuropathology and Experimental Neurology, Lippincott, Williams & Wilkins, 2007, 66 (6), pp.481-90. ⟨10.1097/01.jnen.0000263875.22306.3c⟩ |
| ISSN: | 0022-3069 1554-6578 |
| DOI: | 10.1097/01.jnen.0000263875.22306.3c |
| Popis: | Neurogenesis persists throughout life in the rodent subventricular zone (SVZ) and subgranular zone (SGZ) and increases in the adult after brain injury. In this study, postnatal day 7 rats underwent middle cerebral artery electrocoagulation and transient homolateral common carotid artery occlusion, a lesioning protocol that resulted in ipsilateral (IL) forebrain ischemic injury, leading to a cortical cavity 3 weeks later. The effects of neonatal ischemia on hemispheric damage, cell death, cell proliferation, and neurogenesis were examined 4 hours to 6 weeks later by the terminal deoxynucleotidyl transferase dUTP nick-end labeling assay and immunohistochemistry of Ki-67 in proliferating cells and of doublecortin, a microtubule-associated protein expressed only by immature neurons. Neonatal ischemic injury resulted in persistent reduced IL and transient reduced contralateral (CL) hemispheric areas, a consequence of sustained and transient cell death in the IL and CL areas, respectively. Ki-67 immunostaining revealed 3 peaks of newly generated cells in the dorsal SVZ and SGZ in the IL side and also in the CL side at 48 hours and 7 and 28 days after ischemia. Double immunofluorescence revealed that most of the Ki-67-positive cells were astrocytes at 48 hours. Ischemic injury also stimulated SVZ neurogenesis, based on increased doublecortin immunostaining in both SVZs at 7 to 14 days after injury. Doublecortin-positive neurons remained visible around the lesion at 21 days but displayed an immature shape in discrete chains or clusters. Although unilateral ischemic damage was produced, results indicate successful regenerative changes in the CL hemisphere, allowing anatomical recovery. |
| Databáze: | OpenAIRE |
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