Burkholderia dipogonis sp. nov., isolated from root nodules of Dipogon lignosus in New Zealand and Western Australia
| Autor: | Wendy Y. Y. Liu, Euan K. James, Bruna G. Coutinho, Trevor K. James, John Howieson, Julie Ardley, Mitchell Andrews, Wen-Ming Chen, Shih-Yi Sheu, Ming-Hui Chen, Sofie E. De Meyer |
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| Rok vydání: | 2015 |
| Předmět: |
DNA
Bacterial Root nodule Sequence analysis Burkholderia phytofirmans Burkholderia Ubiquinone Molecular Sequence Data Microbiology Plant Roots Nitrogen Fixation RNA Ribosomal 16S Botany Phaseoleae Ecology Evolution Behavior and Systematics Phospholipids Phylogeny Base Composition biology Fatty Acids Nucleic Acid Hybridization Fabaceae General Medicine Sequence Analysis DNA Western Australia Ribosomal RNA 16S ribosomal RNA biology.organism_classification Bacterial Typing Techniques Burkholderia caledonica Introduced Species New Zealand |
| Zdroj: | International journal of systematic and evolutionary microbiology. 65(12) |
| ISSN: | 1466-5034 |
| Popis: | Seven strains, ICMP 19430T, ICMP 19429, ICMP 19431, WSM4637, WSM4638, WSM4639 and WSM4640, were isolated from nitrogen-fixing nodules on roots of the invasive South African legume Dipogon lignosus (subfamily Papilionoideae, tribe Phaseoleae) in New Zealand and Western Australia, and their taxonomic positions were investigated by using a polyphasic approach. All seven strains grew at 10–37 °C (optimum, 25–30 °C), at pH 4.0–9.0 (optimum, pH 6.0–7.0) and with 0–2 % (w/v) NaCl (optimum growth in the absence of NaCl). On the basis of 16S rRNA gene sequence analysis, the strains showed 99.0–99.5 % sequence similarity to the closest type strain, Burkholderia phytofirmans PsJNT, and 98.4–99.7 % sequence similarity to Burkholderia caledonica LMG 19076T. The predominant fatty acids were C18 : 1ω7c (21.0 % of the total fatty acids in strain ICMP 19430T), C16 : 0 (19.1 %), C17 : 0 cyclo (18.9 %), summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c; 10.7 %) and C19 : 0 cyclo ω8c (7.5 %). The polar lipid profile consisted of a mixture of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and several uncharacterized aminophospholipids and phospholipids. The major isoprenoid quinone was Q-8 and the DNA G+C content of strain ICMP 19430T was 63.2 mol%. The DNA–DNA relatedness of the novel strains with respect to the closest neighbouring members of the genus Burkholderia was 55 % or less. On the basis of 16S rRNA and recA gene sequence similarities and chemotaxonomic and phenotypic data, these strains represent a novel symbiotic species in the genus Burkholderia, for which the name Burkholderia dipogonis sp. nov. is proposed, with the type strain ICMP 19430T ( = LMG 28415T = HAMBI 3637T). |
| Databáze: | OpenAIRE |
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