Recovery of Information Stored in Modified DNA with an Evolved Polymerase

Autor: Raghav Shroff, Jared W. Ellefson, Siyuan S. Wang, Alexander A. Boulgakov, Randall A. Hughes, Andrew D. Ellington
Rok vydání: 2022
Předmět:
Zdroj: ACS synthetic biology. 11(2)
ISSN: 2161-5063
Popis: DNA is increasingly being explored as an alternative medium for digital information storage, but the potential information loss from degradation and associated issues with error during reading challenge its wide-scale implementation. To address this, we propose an atomic-scale encoding standard for DNA, where information is encoded in degradation-resistant analogues of natural nucleic acids (xNAs). To better enable this approach, we used directed evolution to create a polymerase capable of transforming 2'
Databáze: OpenAIRE