Genomic characterization of clinical Enterobacter roggenkampii co-harbouring blaIMP-1- and blaGES-5-encoding IncP6 and mcr-9-encoding IncHI2 plasmids isolated in Japan
| Autor: | Shota Nakamura, Yuki Matsumoto, Akira Fukuda, Hideki Yoshida, Yoshinori Yasui, Kaoru Umeda, Ryuji Kawahara, Hiromi Nakamura, Daisuke Motooka |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Microbiology (medical) mcr-9 IS26 030106 microbiology Immunology Carbapenemase-producing Enterobacterales Biology Microbiology 03 medical and health sciences 0302 clinical medicine Plasmid Enterobacterales blaIMP-1 medicine Immunology and Allergy 030212 general & internal medicine Gene Southern blot bla_ Whole genome sequencing Genetics Whole-genome sequencing Bacterial conjugation Enterobacter biology.organism_classification QR1-502 Colistin blaGES-5 bla_ medicine.drug |
| Zdroj: | Journal of Global Antimicrobial Resistance, Vol 24, Iss, Pp 220-227 (2021) |
| ISSN: | 2213-7165 |
| DOI: | 10.1016/j.jgar.2020.11.028 |
| Popis: | Objectives The spread of carbapenemase-producing Enterobacterales (CPE) with colistin resistance is a critical public health issue. We genetically characterized the clinical isolate Enterobacter roggenkampii OIPH-N260, which harboured carbapenemase genes blaIMP-1 and blaGES-5 with multiple resistance genes, including mcr-9 and blaCTX-M-9. Methods This isolate was characterized by whole-genome sequencing, comparative analysis of resistance plasmids, susceptibility tests, bacterial conjugation, S1-nuclease digested pulsed-field-gel electrophoresis, and Southern blot hybridization. Results The OIPH-N260 isolate exhibited resistance to most β-lactams and colistin. It co-harboured two resistance plasmids, the blaIMP-1- and blaGES-5-encoding IncP6 plasmid pN260-3 and mcr-9- and blaCTX-M-9-encoding IncHI2 plasmid pN260-1. The comparative analysis of pN260-3 indicated that a unique blaIMP-1-surrounding region was inserted into the blaGES-5-encoding plasmid with the mobile element IS26, which plays an important role in the spread of resistance genes. pN260-1 did not possess the mcr-9 expression regulative gene qseBC. Both plasmids were transferable into other bacterial species via conjugation. Conclusions This is the first study to report not only a blaIMP-1 and blaGES-5 co-encoding plasmid, but also the co-harbouring of another plasmid carrying mcr-9 and blaCTX-M-9 in Enterobacter cloacae complex. The development of advanced resistance via IS26-mediated insertion and the co-harbouring of resistance plasmids highlights the need to monitor for resistance genes in CPE. |
| Databáze: | OpenAIRE |
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