Aerobic exercise inhibits obesity-induced respiratory phenotype
| Autor: | Rodolfo de Paula Vieira, Luiz O. Leiria, Fernanda Magalhães Arantes-Costa, Djalma Rabelo Ricardo, Jefferson Comin Jonco Aquino-Junior, Elia Garcia Caldini, Ana Carolina Martins, Mario J.A. Saad, Dioze Guadagnini, Auriléa Aparecida Britto, Ana Roberta Almeida-Oliveira, Manoel Carneiro Oliveira-Junior, BreAnne MacKenzie, Ana Paula Ligeiro de Oliveira, Nilsa Regina Damaceno-Rodrigues |
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| Rok vydání: | 2017 |
| Předmět: |
Male
medicine.medical_specialty Immunology Adipokine Inflammation 030204 cardiovascular system & hematology Diet High-Fat Biochemistry 03 medical and health sciences 0302 clinical medicine Internal medicine Physical Conditioning Animal medicine Respiratory Hypersensitivity Immunology and Allergy Aerobic exercise Animals Obesity Respiratory system Molecular Biology Lung medicine.diagnostic_test Adiponectin business.industry Respiratory disease INTERLEUCINA 10 Hematology respiratory system medicine.disease respiratory tract diseases Elastin Mice Inbred C57BL Bronchoalveolar lavage Endocrinology medicine.anatomical_structure Phenotype 030228 respiratory system Collagen medicine.symptom business Biomarkers |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 1096-0023 |
| Popis: | Obesity results in decreased lung function and increased inflammation. Moderate aerobic exercise (AE) reduced lung inflammation and remodeling in a variety of respiratory disease models. Therefore, this study investigated whether AE can attenuate a diet-induced obesity respiratory phenotype; including airway hyper-responsiveness (AHR), remodeling and inflammation.Sixty C57Bl/6 male mice were distributed into four groups: control lean (CL), exercise lean (EL), obese (O) and obese exercise (OE) groups (2 sets of 7 and 8 mice per group; n = 15). A classical model of diet-induced obesity (DIO) over 12 weeks was used. AE was performed 60 min/day, 5 days/week for 5 weeks. Airway hyperresponsiveness (AHR), lung inflammation and remodeling, adipokines and cytokines in bronchoalveolar lavage (BAL) was determined.A high fat diet over 18 weeks significantly increased body weight (p .0001). Five weeks of AE significantly reduced both AHR and pulmonary inflammation. AHR in obese mice that exercised was reduced at the basal level (p .05), vehicle (PBS) (p .05), 6.25 MCh mg/mL (p .05), 12.5 MCh mg/mL (p .01), 25 MCh mg/mL (p .01) and 50 MCh mg/mL (p .05). Collagen (p .001) and elastic (p .001) fiber deposition in airway wall and also smooth muscle thickness (p .001) were reduced. The number of neutrophils (p .001), macrophages (p .001) and lymphocytes (p .01) were reduced in the peribronchial space as well as in the BAL: lymphocytes (p .01), macrophages (p .01), neutrophils (p .001). AE reduced obesity markers leptin (p .001), IGF-1 (p .01) and VEGF (p .001), while increased adiponectin (p .01) in BAL. AE also reduced pro-inflammatory cytokines in the BAL: IL-1β (p .001), IL-12p40 (p .001), IL-13 (p .01), IL-17 (p .001, IL-23 (p .05) and TNF-alpha (p .05), and increased anti-inflammatory cytokine IL-10 (p .05).Aerobic exercise reduces high fat diet-induced obese lung phenotype (AHR, pulmonary remodeling and inflammation), involving anti-inflammatory cytokine IL-10 and adiponectin. |
| Databáze: | OpenAIRE |
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