Optogenetic Downregulation of Protein Levels with an Ultrasensitive Switch
| Autor: | Lars-Oliver Essen, Jonathan Trauth, Juri Goenrich, Sophia Hasenjäger, Christof Taxis, Sebastian Hepp |
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| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
Light Recombinant Fusion Proteins Biomedical Engineering Arabidopsis Down-Regulation Saccharomyces cerevisiae Optogenetics 01 natural sciences Biochemistry Genetics and Molecular Biology (miscellaneous) 03 medical and health sciences Synthetic biology Downregulation and upregulation Transcription (biology) 010608 biotechnology Drug Resistance Bacterial Transcriptional regulation Transcription factor 030304 developmental biology 0303 health sciences Chemistry Arabidopsis Proteins Protein Stability General Medicine Flow Cytometry Cell biology Luminescent Proteins Doxycycline Synthetic Biology Target protein Degron Genetic Engineering Plasmids |
| Zdroj: | ACS synthetic biology. 8(5) |
| ISSN: | 2161-5063 |
| Popis: | Optogenetic control of protein activity is a versatile technique to gain control over cellular processes, for example, for biomedical and biotechnological applications. Among other techniques, the regulation of protein abundance by controlling either transcription or protein stability found common use as this controls the activity of any type of target protein. Here, we report modules of an improved variant of the photosensitive degron module and a light-sensitive transcription factor, which we compared to doxycycline-dependent transcriptional control. Given their modularity the combined control of synthesis and stability of a given target protein resulted in the synergistic down regulation of its abundance by light. This combined module exhibits very high switching ratios, profound downregulation of protein abundance at low light-fluxes, and fast protein depletion kinetics. Overall, this synergistic optogenetic multistep control (SOMCo) module is easy to implement and results in a regulation of protein abundance superior to each individual component. |
| Databáze: | OpenAIRE |
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