Secretory lipid transfer protein OsLTPL94 acts as a target of EAT1 and is required for rice pollen wall development
| Autor: | Yang Tao, Dan Zhou, Sijing Liu, Yueyang Liang, Jun Zhu, Aijun Wang, Gongwen Li, Pingping Xiong, Menglin Zhou, Zhiyuan He, Qiming Deng, Xu Zhang, Huainian Liu, Shuangcheng Li, Rui Liu, Guoqiang Yuan, Aiping Zheng, Ting Zou, Ping Li, Xiumei Yu, Shiquan Wang, Lingxia Wang, Hao Chen |
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| Rok vydání: | 2021 |
| Předmět: |
Tapetum
Mutation Plant Infertility Somatic cell Mutant Oryza Cell Biology Plant Science Biology medicine.disease_cause Plants Genetically Modified Cell biology E-Box Elements Microspore Gene Expression Regulation Plant Genetics medicine Pollen Carrier Proteins Promoter Regions Genetic Plant lipid transfer proteins Transcription factor Pollen wall Plant Proteins |
| Zdroj: | The Plant journal : for cell and molecular biologyREFERENCES. 108(2) |
| ISSN: | 1365-313X |
| Popis: | The plant pollen wall protects the male gametophyte from various biotic and abiotic stresses. The formation of a unique pollen wall structure and elaborate exine pattern is a well-organized process, which needs coordination between reproductive cells and the neighboring somatic cells. However, molecular mechanisms underlying this process remain largely unknown. Here, we report a rice male-sterile mutant (l94) that exhibits defective pollen exine patterning and abnormal tapetal cell development. MutMap and knockout analyses demonstrated that the causal gene encodes a type-G non-specific lipid transfer protein (OsLTPL94). Histological and cellular analyses established that OsLTPL94 is strongly expressed in the developing microspores and tapetal cells, and its protein is secreted to the plasma membrane. The l94 mutation impeded the secretory ability of OsLTPL94 protein. Further in vivo and in vitro investigations supported the hypothesis that ETERNAL TAPETUM 1 (EAT1), a basic helix-loop-helix transcription factor (bHLH TF), activated OsLTPL94 expression through direct binding to the E-box motif of the OsLTPL94 promoter, which was supported by the positive correlation between the expression of EAT1 and OsLTPL94 in two independent eat1 mutants. Our findings suggest that the secretory OsLTPL94 plays a key role in the coordinated development of tapetum and microspores with the regulation of EAT1. |
| Databáze: | OpenAIRE |
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