Epanorin, a lichen secondary metabolite, inhibits proliferation of MCF-7 breast cancer cells

Autor: Caroline Weinstein-Oppenheimer, M. Fernanda Cavieres, Natalia Quiñones, Flavio Carrión, Hugo A Díaz, Carlos Henríquez-Roldán, Wanda Quilhot, Eduardo de la Peña, Juan Palacios-Moreno, Cecilia Rubio
Přispěvatelé: Comisión Nacional de Investigación Científica y Tecnológica (Chile), Fundación Carolina
Rok vydání: 2019
Předmět:
Zdroj: Biological Research
Digital.CSIC. Repositorio Institucional del CSIC
instname
Biological Research, Vol 52, Iss 1, Pp 1-11 (2019)
Biological Research v.52 2019
SciELO Chile
CONICYT Chile
instacron:CONICYT
Biological Research, Volume: 52, Article number: 55, Published: 28 NOV 2019
ISSN: 0717-6287
DOI: 10.1186/s40659-019-0261-4
Popis: [Background] Epanorin (EP) is a secondary metabolite of the Acarospora lichenic species. EP has been found in lichenic extracts with antimicrobial activity, and UV-absorption properties have been described for closely related molecules; however, its antiproliferative activity in cancer cells has not yet been explored. It has been hypothesized that EP inhibits cancer cell growth. MCF-7 breast cancer cells, normal fibroblasts, and the non-transformed HEK-293 cell line were exposed to increasing concentrations of EP, and proliferation was assessed by the sulforhodamine-B assay.
[Results] MCF-7 cells exposed to EP were examined for cell cycle progression using flow cytometry, and DNA fragmentation was examined using the TUNEL assay. In addition, EP’s mutagenic activity was assessed using the Salmonella typhimurium reverse mutation assay. The data showed that EP inhibits proliferation of MCF-7 cells, and it induces cell cycle arrest in G0/G1 through a DNA fragmentation-independent mechanism. Furthermore, EP’s lack of overt cytotoxicity in the normal cell line HEK-293 and human fibroblasts in cell culture is supported by the absence of mutagenic activity of EP.
[Conclusion] EP emerges as a suitable molecule for further studies as a potential antineoplastic agent.
We acknowledge the support of Centro Regional de Estudios de Alimentos Saludables (CREAS) through Grant R17A10001 for facilities support. This study was supported by grant DIUV 29/2011 to Cecilia Rubio, DIUV-CIDI 10/2017, and a Fellowhip by Fundación Carolina (granted to MF Cavieres).
Databáze: OpenAIRE
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