Megakaryocytic and erythrocytic lineages share specific transcription factors
| Autor: | W. Vainchenker, Gérard Marguerie, Georges Uzan, M. Prenant, Joulin, Marie-Hélène Prandini, Mignotte, Paul-Henri Romeo |
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| Rok vydání: | 1990 |
| Předmět: |
Erythrocytes
Lineage (genetic) Transcription Genetic Gene Expression Biology Polymerase Chain Reaction DNA-binding protein Cell Line NF-E2 Transcription Factor Humans RNA Messenger Binding site Promoter Regions Genetic Transcription factor Gene Messenger RNA Binding Sites Multidisciplinary Base Sequence Erythroid-Specific DNA-Binding Factors Point mutation Serine Endopeptidases DNA Hematopoietic Stem Cells Molecular biology DNA-Binding Proteins NF-E2 Transcription Factor p45 Subunit Mutation Trans-Activators Endopeptidase K Megakaryocytes Transcription Factors |
| Zdroj: | Nature. 344:447-449 |
| ISSN: | 1476-4687 0028-0836 |
| DOI: | 10.1038/344447a0 |
| Popis: | ERYTHROID-specific genes contain binding sites for NF-E1 (also called GF-1 and Eryf-1 ; refs 1–3 respectively), the principal DNA-binding protein of the erythrocytic lineage. NF-E1 expression seems to be restricted to the erythrocytic lineage4. A closely related (if not identical) protein is found in both a human megakaryocytic cell line and purified human megakaryocytes; it binds to promoter regions of two megakaryocytic-specific genes. The binding sites and partial proteolysis profile of this protein are indistinguishable from those of the erythroid protein; also, NF-E1 messenger RNA is the same size in both the megakaryocytic and erythroid cell lines. Furthermore, point mutations that abolish binding of NF-E1 result in a 70% decrease in the transcriptional activity of a megakaryocytic-specific promoter. We also find that NF-E2, another trans5-acting factor of the erythrocytic lineage, is present in megakaryocytes. Transcriptional effects in both lineages might then be mediated in part by the same specific trans-acting factors. Our data strengthen the idea of a close association between the erythrocytic and the megakaryocytic lineages and could also explain the expression of markers specific to the erythrocytic and megakaryocytic lineages in most erythroblastic and megakary-oblastic permanent cell lines5,7. |
| Databáze: | OpenAIRE |
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