Size and location of poly (A) in encephalomyocarditis virus RNA
| Autor: | John C. Smith, David Frisby, Alan George Porter, Valerie Jeffers |
|---|---|
| Rok vydání: | 1976 |
| Předmět: |
chemistry.chemical_classification
Gel electrophoresis Base Sequence biology RNA Sequence (biology) Ribonucleotides Alkaline hydrolysis (body disposal) Molecular biology Virus Molecular Weight Hydrolysis Ribonucleases Biochemistry chemistry Genetics biology.protein RNA Viral Pancreatic ribonuclease Nucleotide RNA Messenger Ribonuclease T1 Encephalomyocarditis virus Poly A |
| Zdroj: | Nucleic Acids Research. 3:2789-2810 |
| ISSN: | 1362-4962 0305-1048 |
| DOI: | 10.1093/nar/3.10.2789 |
| Popis: | Encephalomyocarditis (EMC) virus RNA contains a covalently bound sequence of polyriboadenylic acid (poly(A). This was determined by two-dimensional gel electrophoresis of complete T1 and pancreatic RNase digests of formamidesucrose gradient-purified RNA and subsequent analysis of the product by alkaline hydrolysis. The size of the EMC virus genomic poly(A) sequence was estimated by formamide-polyacrylamide gel electrophoresis of the RNase-resistant product, or by [3H-]poly(U) hybridization to freshly purified virion RNA, to be, on average, 40 nucleotides in length. The evidence obtained from [3H-]isoniazid labelling and other experiments would indicate that the poly(A) sequence is located at the 3'-terminus of EMC virus RNA. |
| Databáze: | OpenAIRE |
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