Localization of the gene transcripts of 11 ?-hydroxylase and aldosterone synthase in the rat adrenal cortex by in situ hybridization
| Autor: | Michihiro Yabu, Hisao Fujita, N. Matsukawa, Y. Nonaka, Takao Senda, Mitsuhiro Okamoto |
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| Rok vydání: | 1991 |
| Předmět: |
Male
Aldosterone synthase endocrine system medicine.medical_specialty Histology medicine.drug_class Biotin Gene Expression chemistry.chemical_compound Cytochrome P-450 Enzyme System Zona fasciculata Corticosterone Internal medicine medicine Animals Cytochrome P-450 CYP11B2 RNA Messenger Steroid 11-beta-hydroxylase Molecular Biology Aldosterone biology Adrenal cortex Nucleic Acid Hybridization Rats Inbred Strains Cell Biology General Medicine Oligonucleotides Antisense Rats Medical Laboratory Technology medicine.anatomical_structure Endocrinology chemistry Zona glomerulosa Mineralocorticoid Steroid Hydroxylases Adrenal Cortex biology.protein Steroid 11-beta-Hydroxylase Zona Glomerulosa Anatomy General Agricultural and Biological Sciences hormones hormone substitutes and hormone antagonists |
| Zdroj: | Histochemistry. 96:391-394 |
| ISSN: | 1432-119X 0301-5564 |
| DOI: | 10.1007/bf00315995 |
| Popis: | Using in situ hybridization, localization of the gene transcripts of 11 beta-hydroxylase and aldosterone synthase was investigated in order to clarify the sites for the synthesis of corticosterone (glucocorticoid) and aldosterone (mineralocorticoid) in the rat adrenal cortex. The gene transcript of 11 beta-hydroxylase was localized in all the endocrine cells of the entire adrenal cortex, while that of aldosterone synthase was exclusively confined in zona glomerulosa cells. These results represent that every endocrine cell of all the cortical zones synthesizes 11 beta-hydroxylase which converts 11-deoxycorticosterone to corticosterone, and only glomerulosa cells synthesize aldosterone synthase which produces aldosterone from corticosterone. Thus it is clearly shown that zona glomerulosa cells synthesize mineralocorticoid, while zona fasciculata as well as reticularis cells produce glucocorticoid. |
| Databáze: | OpenAIRE |
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