Our experience using real-time PCR for the detection of the gene that encodes the superficial lipoprotein LipL32 of the pathogenic leptospires to confirm the acute form of human leptospirosis
| Autor: | Oto Pavlis, Hana Kleprlikova, Zuzana Cermakova, Zbynek Valenta, Lenka Pliskova, Petra Kucerova, Pavla Kubickova |
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| Rok vydání: | 2012 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty Adolescent Lipoproteins Urine Real-Time Polymerase Chain Reaction General Biochemistry Genetics and Molecular Biology Cerebrospinal fluid Blood plasma medicine Humans Leptospirosis Child Aged Aged 80 and over Leptospira medicine.diagnostic_test business.industry Infant Middle Aged medicine.disease Bronchoalveolar lavage Real-time polymerase chain reaction Child Preschool Acute Disease Sputum Female medicine.symptom business Lipoprotein Bacterial Outer Membrane Proteins |
| Zdroj: | Biomedical papers of the Medical Faculty of the University Palacky, Olomouc, Czechoslovakia. 157(4) |
| ISSN: | 1804-7521 |
| Popis: | Aims. To examine biological materials (blood, urine, cerebrospinal fluid) of patients with suspected leptospirosis using real-time PCR for detecting the gene that codes the superficial LipL32 lipoprotein, and to evaluate the contribution of the real-time PCR method for the laboratory diagnosis of the acute form of leptospirosis. Material and Methods. During the monitored period of April 2010 – December 2011, a total of 340 biological materials samples were examined (177x blood plasma, 88x urine, 68x, cerebrospinal fluid, 6x bronchoalveolar lavage and 1x sputum) from 216 patients with suspected leptospirosis using real-time PCR LipL32 gene detection. Results. From the mentioned 216 patients suspected of leptospirosis, 8 patients were evaluated as being PCR LipL32 positive, from which 14 positive biological materials originated (9 x urine, 4x blood and 1x liquor). Conclusion. As demonstrated in the study, the real-time PCR method for detecting the gene for the superficial lipoprotein LipL32 is an appropriate, quick and reliable method for the diagnosis of the acute form of leptospirosis. |
| Databáze: | OpenAIRE |
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